Detailed information of TA system TA190252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 5672633..5674359
• Replicon: chromosome
• Accession: NZ_CP069352
• Organism: Pseudomonas granadensis isolate CT364

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: JN757_RS24850
• Protein ID: WP_203419646.1
• Coordinates: 5672633..5673277 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: JN757_RS24855
• Protein ID: WP_203419647.1
• Coordinates: 5673274..5674359 (+)
• Length: 362 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN757_RS24830	5667909..5669117	-	1209	WP_203419643.1	restriction endonuclease subunit S	-
JN757_RS24835	5669114..5670652	-	1539	WP_203422147.1	type I restriction-modification system subunit M	-
JN757_RS24840	5670941..5671861	+	921	WP_203419644.1	restriction endonuclease	-
JN757_RS24845	5672047..5672694	+	648	WP_203419645.1	DUF4433 domain-containing protein	-
JN757_RS24850	5672633..5673277	+	645	WP_203419646.1	DUF4433 domain-containing protein	Toxin
JN757_RS24855	5673274..5674359	+	1086	WP_203419647.1	macro domain-containing protein	Antitoxin
JN757_RS24860	5674402..5675214	-	813	WP_203419648.1	DUF2135 domain-containing protein	-
JN757_RS24865	5675218..5676837	-	1620	WP_203419649.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 25041.62 Da
• Isoelectric Point: 6.5833

>T190252 WP_203419646.1 NZ_CP069352:5672633-5673277 [Pseudomonas granadensis]
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCANSEVQAPQYVNIGNVDLIDKRRSRQVPIAPEGVLADYVPFYFTPFSV
MMQNIHSGWSVQQRTNDEIIILVSSLYRVVELGLPFVFTNAHAYPDWTDYYRDLTNLDQIDWSILQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCHTDAMKVRIEADVEARGLTLPVHARPKWYFQ

Nucleotide

• Length: 645 bp

>T190252 NZ_CP091198:2033235-2033363 [Enterococcus faecalis]
ATGAAAGGAGCAATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTAT
CTTCGTTATCTTAGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 362 a.a.
• Molecular weight: 40652.66 Da
• Isoelectric Point: 6.6160

>AT190252 WP_203419647.1 NZ_CP069352:5673274-5674359 [Pseudomonas granadensis]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAQNYRVYAAACKAGKVETGKMHVTATDELDGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLLENDVKSVAIPPLGAGNGGLKWPEVREQIVDVLGDLDADVLVFEPSNQYLNVAKRTG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEQLPDTENPLNLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGISDVIWFDQERKAFLQTYLKTEAKDYSQALERTAQLIDGFESPFGMELLATVDWLLNQEGVPATVPAIREA
LKRWDGGAGAAARKSKLFDDQSLEIALKRLATSSFRPELAH

Nucleotide

• Length: 1086 bp

>AT190252 NZ_CP091198:c2033479-2033274 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAA
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC