Detailed information of TA system TA190250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4893679..4894297
• Replicon: chromosome
• Accession: NZ_CP069352
• Organism: Pseudomonas granadensis isolate CT364

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: JN757_RS21355
• Protein ID: WP_197677946.1
• Coordinates: 4893679..4893990 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: JN757_RS21360
• Protein ID: WP_090285453.1
• Coordinates: 4893980..4894297 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN757_RS21330	4889401..4890597	-	1197	WP_203419164.1	hypothetical protein	-
JN757_RS21335	4890679..4891397	-	719	Protein_4194	hypothetical protein	-
JN757_RS21340	4891474..4892289	-	816	WP_203419165.1	sulfite exporter TauE/SafE family protein	-
JN757_RS21345	4892400..4893167	+	768	WP_203419166.1	helix-turn-helix transcriptional regulator	-
JN757_RS21350	4893261..4893578	+	318	WP_203419167.1	XRE family transcriptional regulator	-
JN757_RS21355	4893679..4893990	+	312	WP_197677946.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN757_RS21360	4893980..4894297	+	318	WP_090285453.1	helix-turn-helix domain-containing protein	Antitoxin
JN757_RS21365	4894308..4895363	-	1056	WP_203419168.1	MBL fold metallo-hydrolase	-
JN757_RS21370	4895578..4896186	+	609	WP_203419169.1	TetR/AcrR family transcriptional regulator	-
JN757_RS21375	4896634..4897953	+	1320	WP_203419170.1	PAS domain-containing methyl-accepting chemotaxis protein	-
JN757_RS21380	4898151..4898858	+	708	WP_203419171.1	CTP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4884026..4894297	10271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (29-84)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11341.86 Da
• Isoelectric Point: 7.2118

>T190250 WP_197677946.1 NZ_CP069352:4893679-4893990 [Pseudomonas granadensis]
MPTEVQDVFGHALDLAQCGLKYPDAKPLKGFGGAGVLELIEDFDTNTYRAVYTVRFGCAIYVLHCFQKKSKSGIKTAQSD
IDLIRNRLRAAQDHAKGSESYGD

Nucleotide

• Length: 312 bp

>T190250 NZ_CP091198:c1672951-1672856 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11581.56 Da
• Isoelectric Point: 10.1490

>AT190250 WP_090285453.1 NZ_CP069352:4893980-4894297 [Pseudomonas granadensis]
MEIEVGSGNVYQDLDLPNASEMQVKSQLAAKIGAIIKARHLTQIQASEILGLSQPKLSEMLRGKFRGISEAKMMECLSRL
GRDVQIVVKSAPRSRKEGRIEVVFA

Nucleotide

• Length: 318 bp

>AT190250 NZ_CP091198:1672757-1672821 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT