Detailed information of TA system TA190248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2478582..2479186
• Replicon: chromosome
• Accession: NZ_CP069352
• Organism: Pseudomonas granadensis isolate CT364

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JN757_RS10860
• Protein ID: WP_203421558.1
• Coordinates: 2478893..2479186 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JN757_RS10855
• Protein ID: WP_203421557.1
• Coordinates: 2478582..2478890 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN757_RS10830	2474761..2475780	-	1020	WP_203421553.1	alpha/beta hydrolase	-
JN757_RS10835	2475942..2476841	+	900	WP_203421554.1	LysR family transcriptional regulator	-
JN757_RS10840	2477015..2477287	+	273	WP_090282708.1	ribbon-helix-helix domain-containing protein	-
JN757_RS10845	2477289..2477564	+	276	WP_203421555.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JN757_RS10850	2477664..2478461	+	798	WP_203421556.1	class I SAM-dependent methyltransferase	-
JN757_RS10855	2478582..2478890	-	309	WP_203421557.1	putative addiction module antidote protein	Antitoxin
JN757_RS10860	2478893..2479186	-	294	WP_203421558.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN757_RS10865	2480122..2480814	-	693	WP_203421559.1	N-acetyltransferase	-
JN757_RS10870	2480811..2482001	-	1191	WP_203421560.1	GNAT family N-acetyltransferase	-
JN757_RS10875	2482006..2482695	-	690	WP_203421561.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (23-89)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10683.23 Da
• Isoelectric Point: 10.7543

>T190248 WP_203421558.1 NZ_CP069352:c2479186-2478893 [Pseudomonas granadensis]
MTYLIQQTATFQAWHRSIRDLQAKVAIARRIDRASTGNLGDTKSVGDGVSEMRLDIGPGYRVYFTIRNGAVIILLAGGDK
SSQSADIRRAQKMAKEV

Nucleotide

• Length: 294 bp

>T190248 NZ_CP091198:1178519-1178662 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10964.49 Da
• Isoelectric Point: 6.0606

>AT190248 WP_203421557.1 NZ_CP069352:c2478890-2478582 [Pseudomonas granadensis]
MSQALTNFDMASLLDSDEAISEYLSQVLADGDNEEFLRAIGHVLKARGMTQIAKSSGMGRESLYKAFAPGAKPRFDTVLK
VIHALGMDLCAQPGHVINHSAS

Nucleotide

• Length: 309 bp

>AT190248 NZ_CP091198:c1178852-1178594 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTTAGAAAAATAAGAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATA
ATAGCATTGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCGATTTTGTTACAA
AAAATAACCGTACTCAGTCAAAGTAAACGGTTATTTTTTCTTGTCTTCTTTAACAACCTTTAGGATTCCGAAGATTAAAG
TCGCGATAAAAGAACCGAA