Detailed information of TA system TA190247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2477015..2477564
• Replicon: chromosome
• Accession: NZ_CP069352
• Organism: Pseudomonas granadensis isolate CT364

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JN757_RS10845
• Protein ID: WP_203421555.1
• Coordinates: 2477289..2477564 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JN757_RS10840
• Protein ID: WP_090282708.1
• Coordinates: 2477015..2477287 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN757_RS10820	2472408..2473031	+	624	WP_090282704.1	hydrolase	-
JN757_RS10825	2473282..2474709	-	1428	WP_203421552.1	mechanosensitive ion channel family protein	-
JN757_RS10830	2474761..2475780	-	1020	WP_203421553.1	alpha/beta hydrolase	-
JN757_RS10835	2475942..2476841	+	900	WP_203421554.1	LysR family transcriptional regulator	-
JN757_RS10840	2477015..2477287	+	273	WP_090282708.1	ribbon-helix-helix domain-containing protein	Antitoxin
JN757_RS10845	2477289..2477564	+	276	WP_203421555.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN757_RS10850	2477664..2478461	+	798	WP_203421556.1	class I SAM-dependent methyltransferase	-
JN757_RS10855	2478582..2478890	-	309	WP_203421557.1	putative addiction module antidote protein	-
JN757_RS10860	2478893..2479186	-	294	WP_203421558.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JN757_RS10865	2480122..2480814	-	693	WP_203421559.1	N-acetyltransferase	-
JN757_RS10870	2480811..2482001	-	1191	WP_203421560.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10667.36 Da
• Isoelectric Point: 8.5042

>T190247 WP_203421555.1 NZ_CP069352:2477289-2477564 [Pseudomonas granadensis]
MELKWTSKGLSDLTRLYEFLAAVNQPAAARTVQQLTAAPAMLLANPRLGERLEEFEPRDVRRILVGHYDMRYEIIGPTIY
LLRLWHTREER

Nucleotide

• Length: 276 bp

>T190247 NZ_CP091198:1178519-1178662 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10066.42 Da
• Isoelectric Point: 5.6994

>AT190247 WP_090282708.1 NZ_CP069352:2477015-2477287 [Pseudomonas granadensis]
MAATMKTRSVTAHVPVQLAEKVDLMAERLERSRNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQSVQAWADSL
SSDAPLKAPL

Nucleotide

• Length: 273 bp

>AT190247 NZ_CP091198:c1178408-1178266 [Enterococcus faecalis]
TTGTGCTATAATAGCAATGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTT
TTAGTTACAAAAAATAACCGTACTCAGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAA