Detailed information of TA system TA190245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 141678..143302
• Replicon: chromosome
• Accession: NZ_CP069352
• Organism: Pseudomonas granadensis isolate CT364

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JN757_RS00665
• Protein ID: WP_203420067.1
• Coordinates: 141953..143302 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JN757_RS00660
• Protein ID: WP_090287690.1
• Coordinates: 141678..141956 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN757_RS00630	137097..137720	+	624	WP_203420061.1	FMN-binding negative transcriptional regulator	-
JN757_RS00635	137731..138177	+	447	WP_203420062.1	GNAT family N-acetyltransferase	-
JN757_RS00640	138190..138861	+	672	WP_203420063.1	GNAT family N-acetyltransferase	-
JN757_RS00645	138978..139880	-	903	WP_203420064.1	homocysteine S-methyltransferase family protein	-
JN757_RS00650	139995..140816	+	822	WP_203420065.1	ABC transporter substrate-binding protein	-
JN757_RS00655	140813..141649	+	837	WP_203420066.1	amino acid ABC transporter permease	-
JN757_RS00660	141678..141956	-	279	WP_090287690.1	helix-turn-helix domain-containing protein	Antitoxin
JN757_RS00665	141953..143302	-	1350	WP_203420067.1	HipA domain-containing protein	Toxin
JN757_RS00670	143533..146487	+	2955	WP_203420068.1	autotransporter domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49917.43 Da
• Isoelectric Point: 5.0465

>T190245 WP_203420067.1 NZ_CP069352:c143302-141953 [Pseudomonas granadensis]
MYNLTLQIFTAGIWHDAMVLSFDDPEKGFASRCSFGYETDYLAENIEAIGSPFSKAVSALYPLDWDGRRSETPAFVHDIA
PAGAARRFLLARLGQEKPADISADLYLLGRSTPAPIGNMRIKESAAAVDERKPIGFKREDVIHRDQRFLEYAYELGAAIG
GATGAGGEAPKLLLVENHAGLLYPDAVLSDEDVRQHWFVKFARNRGGLTDQDILRSEFHYYKALQTLGIDTIAEDGLALE
EANKPSLWMHRFDRSVQNGEVSRFAVESIYSLAQVTTPGSAMNHLEVIRLLAGLWRTAGQTSQIPDLVADYLCRDLINKI
LGNSDNHGRNTAIIREADAFRLAPIYDLAPMVMDDEGVTRTTKWPRELERAGDIDWRGVCRALVDIAEPGDPTAVSDESM
LFERLRQEAQRLSALPDILADSGLPARTMNHPGVHLKNLQQRLNDWGLK

Nucleotide

• Length: 1350 bp

>T190245 NZ_CP091198:1173871-1173975 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
GCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10254.91 Da
• Isoelectric Point: 10.9503

>AT190245 WP_090287690.1 NZ_CP069352:c141956-141678 [Pseudomonas granadensis]
MSLSVAERTALIESIQQALAQGTLDIGEAVRRLRVEVTGLHQTQFARMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGM
KMGVVRIHRDNS

Nucleotide

• Length: 279 bp

>AT190245 NZ_CP091198:c1173731-1173577 [Enterococcus faecalis]
TTTTTAGGTAAGTGTGCTATAATAGCAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCGGTTTAAGA
CGGTGACCTTTTGGATTATTTAAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCTTCTTTAA