Detailed information of TA system TA190237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4447921..4449179
• Replicon: chromosome
• Accession: NZ_CP069336
• Organism: Pseudomonas aeruginosa strain E01

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: JQ453_RS20595
• Protein ID: WP_003112528.1
• Coordinates: 4447921..4448676 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JQ453_RS20600
• Protein ID: WP_003086329.1
• Coordinates: 4448811..4449179 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ453_RS20585 (JQ453_20575)	4443094..4445637	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
JQ453_RS20590 (JQ453_20580)	4445792..4447153	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
JQ453_RS20595 (JQ453_20585)	4447921..4448676	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
JQ453_RS20600 (JQ453_20590)	4448811..4449179	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JQ453_RS20605 (JQ453_20595)	4449252..4450538	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
JQ453_RS20610 (JQ453_20600)	4450594..4452088	-	1495	Protein_4092	aldehyde dehydrogenase family protein	-
JQ453_RS20615 (JQ453_20605)	4452263..4452739	+	477	WP_003112531.1	hypothetical protein	-
JQ453_RS20620 (JQ453_20610)	4452742..4453017	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T190237 WP_003112528.1 NZ_CP069336:c4448676-4447921 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190237 NZ_CP091188:26527-26823 [Vibrio anguillarum]
ATGTTCACTATAAAGCGCACGCAACTTTTTGATGATTGGTTGAAGACACTTAAAGATGCTCAGGCACGAGGTGCTATCAC
TGCTCGTGTTCAGCGCCTTACTCAGGGTTTGAGTGGGGATGTTAAGCCGGTTGGTTCGGGCATTAGTGAGCTTAGGATAC
ATGTCGGTAAAGGTTATCGAGTTTATTTTAAGCGTAGTGGAAAACAGATCGTTGTCTTACTTTGCGGTGGCAATAAGAAA
ACTCAGAATCAAGATATCGAACGCGCTAAAGAGTTGGTTCAATATTTTGATTTGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190237 WP_003086329.1 NZ_CP069336:c4449179-4448811 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190237 NZ_CP091188:26847-27137 [Vibrio anguillarum]
ATGAATATTACCAGGCTCGATGATTTTGATCCTGCTGACTATCTTGATAATGCTGAATCTATACAGGCATATATTGATGA
GGCGGTAGCAACTGGTGATGCTGCTTTTATTAGTCAATCCTTGGGTGTTATAGCCCGTGCCAAAGGTATGAATACGCTAG
CAGAGGAAACCGGTTTATCTCGTGAAACTTTATATAGAACATTAAGCGTTAAAGGGAATCCTAACCTTAAAACGCTTATC
TCTATCACTAATGCGCTTGGTGTATCTGTCAAACTCGGCTCTTACTGTTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7