Detailed information of TA system TA190231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4448032..4449290
• Replicon: chromosome
• Accession: NZ_CP069335
• Organism: Pseudomonas aeruginosa strain E02

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: JQ454_RS20600
• Protein ID: WP_003112528.1
• Coordinates: 4448032..4448787 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JQ454_RS20605
• Protein ID: WP_003086329.1
• Coordinates: 4448922..4449290 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ454_RS20590 (JQ454_20585)	4443205..4445748	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
JQ454_RS20595 (JQ454_20590)	4445903..4447264	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
JQ454_RS20600 (JQ454_20595)	4448032..4448787	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
JQ454_RS20605 (JQ454_20600)	4448922..4449290	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JQ454_RS20610 (JQ454_20605)	4449363..4450649	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
JQ454_RS20615 (JQ454_20610)	4450705..4452199	-	1495	Protein_4093	aldehyde dehydrogenase family protein	-
JQ454_RS20620 (JQ454_20615)	4452374..4452850	+	477	WP_003112531.1	hypothetical protein	-
JQ454_RS20625 (JQ454_20620)	4452853..4453128	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T190231 WP_003112528.1 NZ_CP069335:c4448787-4448032 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190231 NZ_CP091186:132991-133278 [Vibrio anguillarum]
ATGACTTATAAGCTAAAATTTCTACCGGCTGCCAAGAAAGAGTGGAGTAAGTTAGCTCCACCAATTCAAAGTCAGTTCAA
AAAGAAGCTTAAAGAGCGTCTGGAAAACCCGCATGTTCCTTCTTCTAAACTGCGTGGCTATGACTCAGTTTATAAGATCA
AACTACGTACAGCAGGCTATCGCTTAGCCTATGAAGTCATTGATGATGAAATCGTGGTGTACGTGTTAGCTATTGGAAAA
CGAGATAAAGATGCAGTGTACAAAAAGTTAGCATCAAGGTTCTCGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190231 WP_003086329.1 NZ_CP069335:c4449290-4448922 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190231 NZ_CP091186:132750-133001 [Vibrio anguillarum]
ATGAGACAGGTTTTAGCTGATTGTTCTGCAAGTATTTCTGAGTTAAAGAAAAATCCAACCGCTTTACTAAACGAAGCTGA
TGGTTCTGCCATTGCTATTCTAAACCACAATAAGCCAGCAGCTTATCTTGTCCCAGCAGAAACTTATGAGTTTCTAATGG
ATATGCTCGATGACTATGAGCTTGCTAAACTAGTTGAAAGTCGCCGTGGTGAATTGTCTGAAGCGGTGGAAGTAAATATC
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7