Detailed information of TA system TA190227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 142974..143479
• Replicon: chromosome
• Accession: NZ_CP069335
• Organism: Pseudomonas aeruginosa strain E02

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: JQ454_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 142974..143255 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: JQ454_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 143252..143479 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ454_RS00635 (JQ454_00635)	138225..139574	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
JQ454_RS00640 (JQ454_00640)	139623..140309	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
JQ454_RS00645 (JQ454_00645)	140410..141144	+	735	WP_003117208.1	GntR family transcriptional regulator	-
JQ454_RS00650 (JQ454_00650)	141324..141734	+	411	WP_003101225.1	aegerolysin family protein	-
JQ454_RS00655 (JQ454_00655)	141766..142674	-	909	WP_010793581.1	LysR family transcriptional regulator	-
JQ454_RS00660 (JQ454_00660)	142974..143255	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
JQ454_RS00665 (JQ454_00665)	143252..143479	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
JQ454_RS00670 (JQ454_00670)	143655..144275	-	621	WP_003101226.1	hypothetical protein	-
JQ454_RS00675 (JQ454_00675)	144376..144876	+	501	WP_003101228.1	LEA type 2 family protein	-
JQ454_RS00680 (JQ454_00680)	144949..145290	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
JQ454_RS00685 (JQ454_00685)	145372..146799	-	1428	WP_003083784.1	GABA permease	-
JQ454_RS00690 (JQ454_00690)	146968..148461	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T190227 WP_003083773.1 NZ_CP069335:c143255-142974 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T190227 NZ_CP091176:2374390-2374746 [Aeromonas caviae]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT190227 WP_003083775.1 NZ_CP069335:c143479-143252 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT190227 NZ_CP091176:2374748-2375083 [Aeromonas caviae]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9