Detailed information of TA system TA190225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4447922..4449180
• Replicon: chromosome
• Accession: NZ_CP069334
• Organism: Pseudomonas aeruginosa strain E03

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: JQ455_RS20590
• Protein ID: WP_003112528.1
• Coordinates: 4447922..4448677 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JQ455_RS20595
• Protein ID: WP_003086329.1
• Coordinates: 4448812..4449180 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ455_RS20580 (JQ455_20580)	4443095..4445638	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
JQ455_RS20585 (JQ455_20585)	4445793..4447154	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
JQ455_RS20590 (JQ455_20590)	4447922..4448677	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
JQ455_RS20595 (JQ455_20595)	4448812..4449180	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JQ455_RS20600 (JQ455_20600)	4449253..4450539	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
JQ455_RS20605 (JQ455_20605)	4450595..4452089	-	1495	Protein_4091	aldehyde dehydrogenase family protein	-
JQ455_RS20610 (JQ455_20610)	4452264..4452740	+	477	WP_003112531.1	hypothetical protein	-
JQ455_RS20615 (JQ455_20615)	4452743..4453018	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T190225 WP_003112528.1 NZ_CP069334:c4448677-4447922 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190225 NZ_CP091175:42411-42695 [Stutzerimonas stutzeri]
GTGCCTGAATTGGAATGGCTAAAATTAGCTCGTGCCGACCTTCTGGCGATTGTTGACTACGTCTCTGATGACAACCCGGA
TGCAGCACAGCGCGTAAAGGATGATATCGAGGCGAAGGCCGAAAAACTGCAAGACTTTCCAGGAATGGGCCGCCCTGGCC
GGGTCGAAGGCACCAGGGAACTCGTGGCCTTGGCGAATTACATTATCGTGTACCAGGAGAGCACTGCTATCGTGCGGATA
CTGCGCGTATTGCATGCAGCGCAGCAGTGGCCATCGTCCGACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190225 WP_003086329.1 NZ_CP069334:c4449180-4448812 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190225 NZ_CP091175:42122-42418 [Stutzerimonas stutzeri]
ATCATGGCTCACACTTCAATGCTGCACGTTCGTGTGGACGACGCGCTGAAAGCTGATGCAGCGAAAAAGCTCGCCAGCTT
CGGCTTGACGGTATCAGATGCCGTTCGCATCCTACTGACTCGCATAACCAAAGAGGGCGGGTTGCCCGTTGGACTAACGA
CTGATCCGGAAGCTCATGACGCCTGGTTCCGCGATAAAGTGAACCAGGCGCTGGCTGACACACGACCTGCCGTTCCTCAT
CAACAGGTCATGGATGAGGCACAAGCGTTGATCGACAGGAAGCGCCGCGGTGCCTGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7