Detailed information of TA system TA190224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3532823..3534044
• Replicon: chromosome
• Accession: NZ_CP069334
• Organism: Pseudomonas aeruginosa strain E03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JQ455_RS16280
• Protein ID: WP_003102740.1
• Coordinates: 3533466..3534044 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQ455_RS16275
• Protein ID: WP_003088112.1
• Coordinates: 3532823..3533377 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ455_RS16250 (JQ455_16260)	3528391..3528942	+	552	WP_004343115.1	XRE family transcriptional regulator	-
JQ455_RS16255 (JQ455_16265)	3528939..3529337	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JQ455_RS16260 (JQ455_16270)	3529350..3529673	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
JQ455_RS16265 (JQ455_16275)	3530004..3530465	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JQ455_RS16270 (JQ455_16280)	3530470..3532665	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JQ455_RS16275 (JQ455_16285)	3532823..3533377	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JQ455_RS16280 (JQ455_16290)	3533466..3534044	+	579	WP_003102740.1	HD domain-containing protein	Toxin
JQ455_RS16285 (JQ455_16295)	3534153..3535340	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JQ455_RS16290 (JQ455_16300)	3535330..3537501	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JQ455_RS16295 (JQ455_16305)	3537491..3538768	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T190224 WP_003102740.1 NZ_CP069334:3533466-3534044 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T190224 NZ_CP091174:c3524495-3524181 [Stutzerimonas stutzeri]
ATGATCTTTATCGAGACTCCGGTCTTTACCAAGCGACTGCGAGAGCTGCTTAACGACGACAGCTATGCAGAGTTTCAGCG
CCAGTTGGCCGAGCGGCCGGAAATGGGTGATGTGATCGAGGGAACCGGCGGCATTCGCAAGGTCCGTGTCGCGACTAGCG
GTCATGGCAAGCGAGGCGGGTCCAGGGTCATCTACTACCACTTCACGGCTGCTTCACAGATCGCGTTGCTGTTGATCTAT
CCGAAGAACGAGAAGGATGACCTGACAGCGGACGAGCGCAAGGTGCTCAAGCAAATCATCGAGCGGTGGAGGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT190224 WP_003088112.1 NZ_CP069334:3532823-3533377 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT190224 NZ_CP091174:c3524175-3523885 [Stutzerimonas stutzeri]
ATGAGCAAATTCTTCGAAGACCTTCTGGAAAGCGTCCAGCAGATGGATGAGATCCATCGAGGCGAGCGGCAACCTTCTCG
GGAATTCACCGTGGATGCGCTGCAGGTGAAGGAGATCCGCAAGGCGACCGGGCTGACTCAAGCCAAGTTCGCGGCCATGA
TCGATGTTCAACTGGGTACGCTGCGTAACTGGGAACAAGGCCGACGCGAACCTACTGGGCCGGCTAAGGCTCTTCTGCGC
GCCATTCATAACGATCCGAAGCACGTCATCCAGGCGCTTTCGTCCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07