Detailed information of TA system TA190219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4386684..4387942
• Replicon: chromosome
• Accession: NZ_CP069333
• Organism: Pseudomonas aeruginosa strain R01

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: JQ457_RS20415
• Protein ID: WP_003112528.1
• Coordinates: 4386684..4387439 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JQ457_RS20420
• Protein ID: WP_003086329.1
• Coordinates: 4387574..4387942 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ457_RS20405 (JQ457_20370)	4381857..4384400	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
JQ457_RS20410 (JQ457_20375)	4384555..4385916	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
JQ457_RS20415 (JQ457_20380)	4386684..4387439	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
JQ457_RS20420 (JQ457_20385)	4387574..4387942	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JQ457_RS20425 (JQ457_20390)	4388015..4389301	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
JQ457_RS20430 (JQ457_20395)	4389357..4390839	-	1483	Protein_4055	aldehyde dehydrogenase family protein	-
JQ457_RS20435 (JQ457_20400)	4391014..4391490	+	477	WP_003112531.1	hypothetical protein	-
JQ457_RS20440 (JQ457_20405)	4391493..4391768	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T190219 WP_003112528.1 NZ_CP069333:c4387439-4386684 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190219 NZ_CP091149:c8154-7846 [Pseudomonas avellanae]
ATGCTGCCGGTGGTCTGGCTCAGTCCCGCACTGGATGATCTGCGCGAAATCGCAACCTACATCGCATGGGAAAACCCGAG
CGCAGCGCGCCGGTTGAAAAGCCTCCTGCAGGAGGCTATCGAGCCGGTAGCCGAACACCCCTATTTATATCGTTCTGGGC
GAGCGCCAGGCACTCGTGAGCTGGTGGCTCACCCAAACTACGTCTTGGTCTATCGTGTCACCCTGGAGCGCATCGAGGTC
GTGAACGTAATCCACGCCAGGCAGGAATACCCGGGGTTTGGGGAGCAATGGAACCGAAAACCAACGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190219 WP_003086329.1 NZ_CP069333:c4387942-4387574 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190219 NZ_CP091149:c8342-8154 [Pseudomonas avellanae]
ATGGCCAACCTCTCACCCATCGTTTCAGAATTCGAAACGGATGAACAGGCCGCAAGCTACGACCGCTGGTTTCGCGCCAA
GGTCGCCGCCAGTCTGGCCGATCCGCGTCCGGCCATCCCTCACGATGAGGTCATGGCGGAAATAGAAGCGCTGATCGATC
AGGTTGAAACCCAACGTGAGAAACGCTGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7