Detailed information of TA system TA190218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3407488..3408709
• Replicon: chromosome
• Accession: NZ_CP069333
• Organism: Pseudomonas aeruginosa strain R01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JQ457_RS15765
• Protein ID: WP_003102740.1
• Coordinates: 3408131..3408709 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQ457_RS15760
• Protein ID: WP_003088112.1
• Coordinates: 3407488..3408042 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ457_RS15735 (JQ457_15720)	3403056..3403607	+	552	WP_004343115.1	XRE family transcriptional regulator	-
JQ457_RS15740 (JQ457_15725)	3403604..3404002	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JQ457_RS15745 (JQ457_15730)	3404015..3404338	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
JQ457_RS15750 (JQ457_15735)	3404669..3405130	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JQ457_RS15755 (JQ457_15740)	3405135..3407330	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JQ457_RS15760 (JQ457_15745)	3407488..3408042	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JQ457_RS15765 (JQ457_15750)	3408131..3408709	+	579	WP_003102740.1	HD domain-containing protein	Toxin
JQ457_RS15770 (JQ457_15755)	3408818..3410005	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JQ457_RS15775 (JQ457_15760)	3409995..3412166	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JQ457_RS15780 (JQ457_15765)	3412156..3413433	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T190218 WP_003102740.1 NZ_CP069333:3408131-3408709 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T190218 NZ_CP091149:2063-2374 [Pseudomonas avellanae]
ATGCTACCCATTTTCTGGCTAGAATCAGCGGACAACGATTTGGCGGCAATAATCGAATACATAGGTTTACGTGATATAGC
TGCTGCCGAAAGGTTGTGGCAGCGCTTGCGTGGCATCGTTCTGCCCCTTTCAGAACATCCTTATCTGTATGCGATAAGTG
ACCGCGTTCCAGGAATGCGCGAGATCGTGGCCCATCCTAACTACCTGGTGTTTTATCGCGTCACCTCAACGCGCATAGAA
GTGGTGAACGTCGTCCACGCACGGCAAGAATATCCCCAAACTGGACTAGGGTCTGTTCCCGTTTCGACGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT190218 WP_003088112.1 NZ_CP069333:3407488-3408042 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT190218 NZ_CP091149:1878-2063 [Pseudomonas avellanae]
ATGATTGATCGTTCGCCTATCGTTTCGGAGTTTGAAACTGAAGAGCTAGAGGCCAATTACACCGTCTGGCTACGCGCGAA
GGTGGAGGCAAGTCTGGCCGACTCTCGCCCTGCTATCCCGCATGATGAAGTCGAGCGAAGGATGGCAGAGCGGTTGGCAC
GATTGCGCCATCGGCGAGCGTCCTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07