Detailed information of TA system TA190216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1568762..1570457
• Replicon: chromosome
• Accession: NZ_CP069333
• Organism: Pseudomonas aeruginosa strain R01

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: JQ457_RS07315
• Protein ID: WP_003112901.1
• Coordinates: 1568762..1569631 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: JQ457_RS07320
• Protein ID: WP_003112902.1
• Coordinates: 1569624..1570457 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ457_RS07290 (JQ457_07275)	1564770..1565621	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
JQ457_RS07295 (JQ457_07280)	1565662..1566669	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
JQ457_RS07300 (JQ457_07285)	1566666..1567442	+	777	WP_003112898.1	ABC transporter permease	-
JQ457_RS07305 (JQ457_07290)	1567448..1568209	+	762	WP_003112899.1	SDR family oxidoreductase	-
JQ457_RS07310 (JQ457_07295)	1568223..1568753	+	531	WP_003112900.1	cupin domain-containing protein	-
JQ457_RS07315 (JQ457_07300)	1568762..1569631	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
JQ457_RS07320 (JQ457_07305)	1569624..1570457	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
JQ457_RS07325 (JQ457_07310)	1570444..1571241	+	798	WP_003112903.1	SDR family oxidoreductase	-
JQ457_RS07330 (JQ457_07315)	1571234..1572916	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
JQ457_RS07335 (JQ457_07320)	1572927..1573730	+	804	WP_003130116.1	aspartate dehydrogenase	-
JQ457_RS07340 (JQ457_07325)	1573742..1575226	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T190216 WP_003112901.1 NZ_CP069333:1568762-1569631 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T190216 NZ_CP091148:64943-65269 [Pseudomonas avellanae]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACCCTCAAGCGTGAAAAA
ATAGGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTAGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT190216 WP_003112902.1 NZ_CP069333:1569624-1570457 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT190216 NZ_CP091148:64722-64946 [Pseudomonas avellanae]
ATGCCTACATCCCATGTAAATGCCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1