Detailed information of TA system TA190214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 5179060..5180755
• Replicon: chromosome
• Accession: NZ_CP069332
• Organism: Pseudomonas aeruginosa strain R08

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: JQ464_RS24075
• Protein ID: WP_003112901.1
• Coordinates: 5179886..5180755 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: JQ464_RS24070
• Protein ID: WP_003112902.1
• Coordinates: 5179060..5179893 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ464_RS24050 (JQ464_24025)	5174291..5175775	-	1485	WP_003453562.1	aldehyde dehydrogenase	-
JQ464_RS24055 (JQ464_24030)	5175787..5176590	-	804	WP_003130116.1	aspartate dehydrogenase	-
JQ464_RS24060 (JQ464_24035)	5176601..5178283	-	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
JQ464_RS24065 (JQ464_24040)	5178276..5179073	-	798	WP_003112903.1	SDR family oxidoreductase	-
JQ464_RS24070 (JQ464_24045)	5179060..5179893	-	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
JQ464_RS24075 (JQ464_24050)	5179886..5180755	-	870	WP_003112901.1	alpha/beta hydrolase	Toxin
JQ464_RS24080 (JQ464_24055)	5180764..5181294	-	531	WP_003112900.1	cupin domain-containing protein	-
JQ464_RS24085 (JQ464_24060)	5181308..5182069	-	762	WP_003112899.1	SDR family oxidoreductase	-
JQ464_RS24090 (JQ464_24065)	5182075..5182851	-	777	WP_003112898.1	ABC transporter permease	-
JQ464_RS24095 (JQ464_24070)	5182848..5183855	-	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
JQ464_RS24100 (JQ464_24075)	5183896..5184747	-	852	WP_003130104.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T190214 WP_003112901.1 NZ_CP069332:c5180755-5179886 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T190214 NZ_CP091147:4596339-4596578 [Pseudomonas avellanae]
CTGGGGCATACCGTTAGTGCTCAACTCAAGAAAAAGCTTTTGGAGCGGCTCGAATTACCTCGAAATGCGGGCGATGCGCT
GCATGGCATGCCCGATCACTACAAAATCAAGTTGCGCAGTGCTGGATACAGACTGGTTTACCGGGTGGAAGATGATCGGG
TGGTAGTGACCGTCGTCGCTGTAGGCAAGCGCGAACGAGGGGATATCTACGATTCAGCCAAGGACAGGCTGTCTCCCTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT190214 WP_003112902.1 NZ_CP069332:c5179893-5179060 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT190214 NZ_CP091147:4596053-4596298 [Pseudomonas avellanae]
ATGCAAAATATCTTTGCGGACATCGCGGTCAGCGTCTCTGAACTGAAGAAAAACCCGTCAGCCGTCCTGAGCGGCGCACA
AGGCTTGCCCGTCGCAGTACTCAATCACAATAAAGTCATGGGCTACATGGTGCCTGCCGAGGTGTTTGAAGCAATGATGG
AGCGCCTTGATGACCTCGATCTGGCCGACCTGATCAAAGCCAGGCAACATGAAAAGCCGGTATCGGTAGATATACTTGAC
CTTTAA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1