Detailed information of TA system TA190212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3340896..3342117
• Replicon: chromosome
• Accession: NZ_CP069332
• Organism: Pseudomonas aeruginosa strain R08

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JQ464_RS15630
• Protein ID: WP_003102740.1
• Coordinates: 3340896..3341474 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQ464_RS15635
• Protein ID: WP_003088112.1
• Coordinates: 3341563..3342117 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ464_RS15615 (JQ464_15580)	3336172..3337449	+	1278	WP_003102743.1	TolC family protein	-
JQ464_RS15620 (JQ464_15585)	3337439..3339610	+	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JQ464_RS15625 (JQ464_15590)	3339600..3340787	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JQ464_RS15630 (JQ464_15595)	3340896..3341474	-	579	WP_003102740.1	HD domain-containing protein	Toxin
JQ464_RS15635 (JQ464_15600)	3341563..3342117	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JQ464_RS15640 (JQ464_15605)	3342275..3344470	-	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JQ464_RS15645 (JQ464_15610)	3344475..3344936	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JQ464_RS15650 (JQ464_15615)	3345267..3345590	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
JQ464_RS15655 (JQ464_15620)	3345603..3346001	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JQ464_RS15660 (JQ464_15625)	3345998..3346549	-	552	WP_004343115.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T190212 WP_003102740.1 NZ_CP069332:c3341474-3340896 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T190212 NZ_CP091147:4514470-4514751 [Pseudomonas avellanae]
ATGAGAGAGTTGAAGGTTTTCGAAGCTTCTGACTACCTGAACAACGACGAGGAAATTGCCGAGTTTCTGAGCATTTCCCT
GGAAGACCCAAACCCTGACGTTTTCCTCAGGGCTTTGCAGGAAGTCGCGAAGGCGCGTGGAATGAGCCAGCTTGCCCGTG
ACGCTGGCATCAATCGCGAATCGCTCTACAAAGCTTTGACGCCAGGTGCAAAGCCTCGGTATGACACGGTTTTGAAGGTG
ATGAATGCGTTAGGTATGAAGCTGACCGCCCAGCACGTATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT190212 WP_003088112.1 NZ_CP069332:c3342117-3341563 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT190212 NZ_CP091147:4514165-4514473 [Pseudomonas avellanae]
ATGAACACACTCATCAGAACCCACGAATTCGACGCATGGCTGAAAGGCTTGAAGGACGTAACGGCGCGCCTGAGAATCCT
GAACCGTATCATGGCGGCACAGGAAGGCAACTTTGGGGACTGCGAACCTGTCGGGCACGGCATCAGTGAAATGCGCATCT
TCGTAGGACCTGGCTACCGTGTGTACTACACCAGGCGCGCAGAAGTGGTCTATCTGCTACTGTGTGGCGGCAACAAGTCC
TCACAGAAGCGCGACATAAAGCTGGCGAAATCCATCCTCGATGAGATCGAGAAAGGAGACGTAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07