Detailed information of TA system TA190195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3340557..3341778
• Replicon: chromosome
• Accession: NZ_CP069329
• Organism: Pseudomonas aeruginosa strain R10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JQ466_RS15630
• Protein ID: WP_003102740.1
• Coordinates: 3340557..3341135 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQ466_RS15635
• Protein ID: WP_003088112.1
• Coordinates: 3341224..3341778 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ466_RS15615 (JQ466_15580)	3335833..3337110	+	1278	WP_003102743.1	TolC family protein	-
JQ466_RS15620 (JQ466_15585)	3337100..3339271	+	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JQ466_RS15625 (JQ466_15590)	3339261..3340448	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JQ466_RS15630 (JQ466_15595)	3340557..3341135	-	579	WP_003102740.1	HD domain-containing protein	Toxin
JQ466_RS15635 (JQ466_15600)	3341224..3341778	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JQ466_RS15640 (JQ466_15605)	3341936..3344131	-	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JQ466_RS15645 (JQ466_15610)	3344136..3344597	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JQ466_RS15650 (JQ466_15615)	3344928..3345251	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
JQ466_RS15655 (JQ466_15620)	3345264..3345662	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JQ466_RS15660 (JQ466_15625)	3345659..3346210	-	552	WP_004343115.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T190195 WP_003102740.1 NZ_CP069329:c3341135-3340557 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T190195 NZ_CP091143:4600689-4600871 [Pseudomonas avellanae]
ATGAAGTGCAGCGAGTTTCGGCGATGGTTACAGGCTCAAGGCGCTGAGTTCAAAGCGGCCAAGGGCAGCCACTTCAAGGT
GTACTTGAATGGCAAGGCGACCATCTTTGCGGACCACGGCAGCAAGGAAATGCACGAAGGGTTACGTAAATCGATCATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT190195 WP_003088112.1 NZ_CP069329:c3341778-3341224 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT190195 NZ_CP091143:4600904-4601329 [Pseudomonas avellanae]
ATGTATCAGTACCCGTTAGAACTGCATCAGGAACCAACCGGCATCTGGTTGTCATGCCCGGATATTCCCGAAATGAACGC
CTCGGGTGACACCTTGGACGAGGCGCTGGCCGAAGCCCTGAACGGCATGGAGTCGGCCTTTTCGCTCTACGTTGACCAGC
GCCGCAAGATCCCGCAGGCGTCGCTGCCATCCGGCGATGAGTTGGTGATGCACCTGCCTGCGCTGACTGTCGCCAAGATC
ATGCTGTGGAACAGCATGTTGGACAATGGCGTGAGCCGGGCCGAACTGGCGCGCCGTCTGAGCTGTACGCGGCAAGTTGT
GGATCGGCTGGTGGACTTCCTGCACACCTCGAAAATCGAGCAGGTGGAGCGGGCATTGGCTGTGCTGGGTCGGCGCATAA
CGTTGAGCATTGAGCAGGCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07