Detailed information of TA system TA190194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 2361559..2362817
• Replicon: chromosome
• Accession: NZ_CP069329
• Organism: Pseudomonas aeruginosa strain R10

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: JQ466_RS10990
• Protein ID: WP_003112528.1
• Coordinates: 2362062..2362817 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JQ466_RS10985
• Protein ID: WP_003086329.1
• Coordinates: 2361559..2361927 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ466_RS10965 (JQ466_10950)	2357722..2357997	+	276	WP_010793902.1	zinc ribbon domain-containing protein	-
JQ466_RS10970 (JQ466_10955)	2358000..2358476	-	477	WP_003112531.1	hypothetical protein	-
JQ466_RS10975 (JQ466_10960)	2358651..2360144	+	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
JQ466_RS10980 (JQ466_10965)	2360200..2361486	+	1287	WP_003112529.1	FAD-binding oxidoreductase	-
JQ466_RS10985 (JQ466_10970)	2361559..2361927	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JQ466_RS10990 (JQ466_10975)	2362062..2362817	+	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
JQ466_RS10995 (JQ466_10980)	2363585..2364946	+	1362	WP_010895536.1	DNA recombination protein RmuC	-
JQ466_RS11000 (JQ466_10985)	2365101..2367644	-	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T190194 WP_003112528.1 NZ_CP069329:2362062-2362817 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190194 NZ_CP091143:c4345709-4345419 [Pseudomonas avellanae]
ATGACTTATAGCCTCGAATTTGATGCGCGAGCTCTGAAGGAATGGCACAAATTAGGGGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCAAATCGCCTGCATGCGTTGCCCGACTGCTACAAAATCA
AGCTGCGCAGCAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAACAGGTTGTGGTCTTCGTGGTTGCTGTGGACAAG
CGTGAGCGAGATGAGGTTTACCGTAAGGCGGCTGATCGGTTGGGTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190194 WP_003086329.1 NZ_CP069329:2361559-2361927 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190194 NZ_CP091143:c4345938-4345699 [Pseudomonas avellanae]
ATGGCGTCTATTAATGTTCGTATCGACGACGATCTCAAGGCTCGTGCTTATCTCGAGCTTGAAAAGCTTGGCGTCACTCC
TTCGGAACTGCTGCGTCAAACCCTGCAGTACGTGGCAGAGCGCGGCCAGCTACCGTTCAAGACCGTACTGATGACCGAGG
AAGACGAAGTCTTGCTTGCAACAGTGCGCGAGCGCCTTGCGGCTCCGCAACGGGTCAAGGTTTCACTGGATGACTTATAG

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7