Detailed information of TA system TA190189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3407169..3408390
• Replicon: chromosome
• Accession: NZ_CP069328
• Organism: Pseudomonas aeruginosa strain R04

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JQ460_RS15750
• Protein ID: WP_003102740.1
• Coordinates: 3407812..3408390 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQ460_RS15745
• Protein ID: WP_003088112.1
• Coordinates: 3407169..3407723 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ460_RS15720 (JQ460_15720)	3402737..3403288	+	552	WP_004343115.1	XRE family transcriptional regulator	-
JQ460_RS15725 (JQ460_15725)	3403285..3403683	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JQ460_RS15730 (JQ460_15730)	3403696..3404019	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
JQ460_RS15735 (JQ460_15735)	3404350..3404811	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JQ460_RS15740 (JQ460_15740)	3404816..3407011	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JQ460_RS15745 (JQ460_15745)	3407169..3407723	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JQ460_RS15750 (JQ460_15750)	3407812..3408390	+	579	WP_003102740.1	HD domain-containing protein	Toxin
JQ460_RS15755 (JQ460_15755)	3408499..3409686	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JQ460_RS15760 (JQ460_15760)	3409676..3411847	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JQ460_RS15765 (JQ460_15765)	3411837..3413114	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T190189 WP_003102740.1 NZ_CP069328:3407812-3408390 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T190189 NZ_CP091139:2077189-2077623 [Microbacterium sp. KUDC0405]
ATGAAGCTCGTCGACACGAACGTTCTCGTGTACGCCGTGGTCTCGGATGCGCCGCACCATGCGCGTTCGAAGGAGTGGCT
CGAGCGCGCGTTCGGGGGCGTTGACACCGTTCTGCTGCCGTGGGTGAGTCTTATCGGCTTCATCCGGATTGTGAGCAATC
CGCGTATCCACGAGAGCCCCGTTGCCGTGGATGAGGCACTGCGGGTGGTCGACGAATGGCTGGAGCGCGCGGTATCCGTC
GCTCCCGTACCGGACGCGCGCCATCCCCATCGCATGCGCGAATTGCTCACTCCGATCGCCGCGTCGGGCGGGAACATCGT
CAACGACGCTCATCTGGCAGCGCTTGCCGTCCAGTACGGCGCAACAGTCGTGACGTTCGATTCAGACTTCGGCCGGTTCC
CCGGGGTGAAGTGGGAGCAACCGGGTCCGGGCTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT190189 WP_003088112.1 NZ_CP069328:3407169-3407723 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT190189 NZ_CP091139:2076944-2077192 [Microbacterium sp. KUDC0405]
ATGCGCACCACCATCACGTTGACGCCCGAGGCCGAGTCGGTGATCCGCCGCATGATGGACGAGCGGAAGCTGTCGTTCAA
AGAGGCTGTGAACTCCGCCATTCTCAGTGCGCCACGCCATGAGGCACGCGAACGATACGTGCTGCCCTCCTTCACCCTCG
GCACGCGGGTCCCATTGGATGACATCAAGGGTCTGCTCGGGCAACTCGACGACGAGGACTACCTGCGCAAGCAGGAGTTC
GGCAAATGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07