Detailed information of TA system TA190186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 142962..143467
• Replicon: chromosome
• Accession: NZ_CP069328
• Organism: Pseudomonas aeruginosa strain R04

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: JQ460_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 142962..143243 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: JQ460_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 143240..143467 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ460_RS00635 (JQ460_00635)	138213..139562	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
JQ460_RS00640 (JQ460_00640)	139611..140297	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
JQ460_RS00645 (JQ460_00645)	140398..141132	+	735	WP_003117208.1	GntR family transcriptional regulator	-
JQ460_RS00650 (JQ460_00650)	141312..141722	+	411	WP_003101225.1	aegerolysin family protein	-
JQ460_RS00655 (JQ460_00655)	141754..142662	-	909	WP_010793581.1	LysR family transcriptional regulator	-
JQ460_RS00660 (JQ460_00660)	142962..143243	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
JQ460_RS00665 (JQ460_00665)	143240..143467	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
JQ460_RS00670 (JQ460_00670)	143643..144263	-	621	WP_003101226.1	hypothetical protein	-
JQ460_RS00675 (JQ460_00675)	144364..144864	+	501	WP_003101228.1	LEA type 2 family protein	-
JQ460_RS00680 (JQ460_00680)	144937..145278	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
JQ460_RS00685 (JQ460_00685)	145360..146787	-	1428	WP_003083784.1	GABA permease	-
JQ460_RS00690 (JQ460_00690)	146956..148449	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T190186 WP_003083773.1 NZ_CP069328:c143243-142962 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T190186 NZ_CP091139:c1560124-1559753 [Microbacterium sp. KUDC0405]
ATGACCGGCGACGGCGTCACGCTCGTCCGCCGCGGGGCGATCGTGCTGGTCGACCTCGATCCGGTCTCCGGTTCCGAGGC
GTCCAAGACGCGACCCGCGGTCGTGGTGAGCAACAACACCGCGAATGCGGCCGCGGTGCGGACGCGGCGCGGCGTGATCA
CGGTCGTGCCGATCACGAGCAGCACCGCACGGGTGTTCCCGTTCCACGTCCTCCTCGACGCCGATGCGACCGGCCTCAGC
CACGACTCGAAGGCGCAGGCCGAACAGGTGCGTGCCGTGTCGATCGGGCGGATCCTGCGCATCGTCGGCTGGGTTCCCGC
CGAGGCGATGACGCAGGTGGATGCCGCACTGCGTCTTCACCTCGCGCTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT190186 WP_003083775.1 NZ_CP069328:c143467-143240 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT190186 NZ_CP091139:c1560345-1560121 [Microbacterium sp. KUDC0405]
ATGGGCGCCAAGGTGAGCATCAGCTTGGGTGAGGACGACATCGCCGCACTCGATGCGGACGTCCTCGCCGGCCGGTTCGC
GAGCCGCTCCGCCGCGGTGCAGCGGGCGCTTCAGCTGCTGCGTGAGAGCGAGCTCGCCGATGCCTACGCGGAGGCGTTCG
GCTCGTGGGTGGATGACGGTGAGGCGGCCGCCTGGGATGCGGCGCCAGACGACGGGGTCGCATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9