Detailed information of TA system TA190184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4386681..4387939
• Replicon: chromosome
• Accession: NZ_CP069327
• Organism: Pseudomonas aeruginosa strain R03

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: JQ459_RS20400
• Protein ID: WP_003112528.1
• Coordinates: 4386681..4387436 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JQ459_RS20405
• Protein ID: WP_003086329.1
• Coordinates: 4387571..4387939 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ459_RS20390 (JQ459_20365)	4381854..4384397	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
JQ459_RS20395 (JQ459_20370)	4384552..4385913	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
JQ459_RS20400 (JQ459_20375)	4386681..4387436	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
JQ459_RS20405 (JQ459_20380)	4387571..4387939	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JQ459_RS20410 (JQ459_20385)	4388012..4389298	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
JQ459_RS20415 (JQ459_20390)	4389354..4390847	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
JQ459_RS20420 (JQ459_20395)	4391022..4391498	+	477	WP_003112531.1	hypothetical protein	-
JQ459_RS20425 (JQ459_20400)	4391501..4391776	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T190184 WP_003112528.1 NZ_CP069327:c4387436-4386681 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190184 NZ_CP091138:373234-373647 [Microbacterium sp. KUDC0406]
GTGTACCTGCTCGATACCAACGCGTATTCCGAATCACGTCGCCCCGGCTTGCTCGGTGAGACCATGCGGACCTGGCTCGC
AGACACGCCTCCCGAAGTCACGTTCATGAGTGCGATCACCGACTACGAGCTGGAACGCGGCGTGCTGCATCTGCAACGTC
GAGATCCGCGCCAGGCGACAGCGCTACGTGGTTGGTTGACGACCGTGCGATCGGGTCTGGAAGGTCGCATCCTGTCGGTC
ACTCCGGAGATCGCGAGGGTCTTCGCACGGTTGGACGTACCCGACCGTCGACCATGGGCGGATGCGCTGATCGCCGCCAC
CGCTCTTCACCACGACCTGACCGTCGTCACTCGGAACGAGAAGGACTTCGACGTGCCGGGGCTGCGGGTGATCAACCCGT
TCTCAGGTGATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190184 WP_003086329.1 NZ_CP069327:c4387939-4387571 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190184 NZ_CP091138:373777-374049 [Microbacterium sp. KUDC0406]
GTGATCGAAGACATCAAGAAGCGTGCTCTGCACCGCACCAGCATCCTCGAGGGGCAGCTGCGCGGCATCGCGCGGATGAT
CGAGAACGACGAGTACTGCATGGACATCATCACCCAGTCCAGGGCCGTCCAGCGCTCTCTCGAGTCGCTGAACCGACTGC
TTCTCGAGAACCACCTGCGCACCCACGTGACGGACATGTTCGAGGAGGGCGGCGAACAGCGGGAGCAGGCCGTCGACGAA
CTGCTGAAGGCGTTCGACTTCCACTCGAAATGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7