Detailed information of TA system TA190183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3407468..3408689
• Replicon: chromosome
• Accession: NZ_CP069327
• Organism: Pseudomonas aeruginosa strain R03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JQ459_RS15760
• Protein ID: WP_003102740.1
• Coordinates: 3408111..3408689 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQ459_RS15755
• Protein ID: WP_003088112.1
• Coordinates: 3407468..3408022 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ459_RS15730 (JQ459_15720)	3403036..3403587	+	552	WP_004343115.1	XRE family transcriptional regulator	-
JQ459_RS15735 (JQ459_15725)	3403584..3403982	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JQ459_RS15740 (JQ459_15730)	3403995..3404318	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
JQ459_RS15745 (JQ459_15735)	3404649..3405110	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JQ459_RS15750 (JQ459_15740)	3405115..3407310	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JQ459_RS15755 (JQ459_15745)	3407468..3408022	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JQ459_RS15760 (JQ459_15750)	3408111..3408689	+	579	WP_003102740.1	HD domain-containing protein	Toxin
JQ459_RS15765 (JQ459_15755)	3408798..3409985	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JQ459_RS15770 (JQ459_15760)	3409975..3412146	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JQ459_RS15775 (JQ459_15765)	3412136..3413413	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T190183 WP_003102740.1 NZ_CP069327:3408111-3408689 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T190183 NZ_CP091138:c187254-186826 [Microbacterium sp. KUDC0406]
ATGATCGTCGTCGACGTCAACGTGCTGATCGCGGCGTTCCGCCAGGATCATCCGCATCACGATGTCGCGCTCCCGTGGCT
CTCACAGGCGCTCTCGGGTTCCGACGACATCGTCGTCCCGGATGCGGTATGGACCGGGTTCCTGCGGATCGTCACTCTGC
CGGGAGCCTTCCGCGTTCCCTCGAGGTTGGACGAGGCTTCGGCCTTCATCCACGATGTGGTGTCTGCGGGCGCGTACCTC
ACCGCTGCGCCGTTGATCGACGGAGCGACGCACCTGGTCGCGTTGTGCATCGAGACGCAGGCGACCGGCAATCTCGTACC
GGATGCGTACATCGCATCGGTCGCGCTGGGCTACGACTGCCCGGTGGCGACCTTCGACCGCGACTTCCGCCGCTTCGACG
GACTGCGGATCATCACTCCTGCCGCGTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT190183 WP_003088112.1 NZ_CP069327:3407468-3408022 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT190183 NZ_CP091138:c187493-187251 [Microbacterium sp. KUDC0406]
ATGCGCACCACCCTCTCGATCAACGATCGCCTGCTCGCGCAGGCGAAGGCCCGCGCCCAGGAGTCCGGCGTGACGCTCGG
CGCTTATGTCGAAGAGGTGCTGCGGCGCGATCTCGCGGCATCTCGGCCTGCCCGACGACCAGTGACTCTGACCGTGTCAG
CAGCGACCGGCGGCGCCCAGCCCGGCGTCGATCTCACCACGAATCGCGGTCTGTACGAGGCGATGGGCACCGAGATCGCA
TGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07