Detailed information of TA system TA190181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1568783..1570478
• Replicon: chromosome
• Accession: NZ_CP069327
• Organism: Pseudomonas aeruginosa strain R03

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: JQ459_RS07315
• Protein ID: WP_003112901.1
• Coordinates: 1568783..1569652 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: JQ459_RS07320
• Protein ID: WP_003112902.1
• Coordinates: 1569645..1570478 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ459_RS07290 (JQ459_07275)	1564791..1565642	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
JQ459_RS07295 (JQ459_07280)	1565683..1566690	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
JQ459_RS07300 (JQ459_07285)	1566687..1567463	+	777	WP_003112898.1	ABC transporter permease	-
JQ459_RS07305 (JQ459_07290)	1567469..1568230	+	762	WP_003112899.1	SDR family oxidoreductase	-
JQ459_RS07310 (JQ459_07295)	1568244..1568774	+	531	WP_003112900.1	cupin domain-containing protein	-
JQ459_RS07315 (JQ459_07300)	1568783..1569652	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
JQ459_RS07320 (JQ459_07305)	1569645..1570478	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
JQ459_RS07325 (JQ459_07310)	1570465..1571262	+	798	WP_003112903.1	SDR family oxidoreductase	-
JQ459_RS07330 (JQ459_07315)	1571255..1572937	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
JQ459_RS07335 (JQ459_07320)	1572948..1573751	+	804	WP_003130116.1	aspartate dehydrogenase	-
JQ459_RS07340 (JQ459_07325)	1573763..1575247	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T190181 WP_003112901.1 NZ_CP069327:1568783-1569652 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T190181 NZ_CP091127:c148360-148073 [Serratia marcescens]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT190181 WP_003112902.1 NZ_CP069327:1569645-1570478 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT190181 NZ_CP091127:c148599-148360 [Serratia marcescens]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1