Detailed information of TA system TA190180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 142953..143458
• Replicon: chromosome
• Accession: NZ_CP069327
• Organism: Pseudomonas aeruginosa strain R03

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: JQ459_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 142953..143234 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: JQ459_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 143231..143458 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ459_RS00635 (JQ459_00635)	138204..139553	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
JQ459_RS00640 (JQ459_00640)	139602..140288	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
JQ459_RS00645 (JQ459_00645)	140389..141123	+	735	WP_003117208.1	GntR family transcriptional regulator	-
JQ459_RS00650 (JQ459_00650)	141303..141713	+	411	WP_003101225.1	aegerolysin family protein	-
JQ459_RS00655 (JQ459_00655)	141745..142653	-	909	WP_010793581.1	LysR family transcriptional regulator	-
JQ459_RS00660 (JQ459_00660)	142953..143234	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
JQ459_RS00665 (JQ459_00665)	143231..143458	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
JQ459_RS00670 (JQ459_00670)	143634..144254	-	621	WP_003101226.1	hypothetical protein	-
JQ459_RS00675 (JQ459_00675)	144355..144855	+	501	WP_003101228.1	LEA type 2 family protein	-
JQ459_RS00680 (JQ459_00680)	144928..145269	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
JQ459_RS00685 (JQ459_00685)	145351..146778	-	1428	WP_003083784.1	GABA permease	-
JQ459_RS00690 (JQ459_00690)	146947..148440	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T190180 WP_003083773.1 NZ_CP069327:c143234-142953 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T190180 NZ_CP069327:c143234-142953 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT190180 WP_003083775.1 NZ_CP069327:c143458-143231 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT190180 NZ_CP069327:c143458-143231 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9