Detailed information of TA system TA190166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4386403..4387661
• Replicon: chromosome
• Accession: NZ_CP069324
• Organism: Pseudomonas aeruginosa strain R05

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: JQ461_RS20395
• Protein ID: WP_003112528.1
• Coordinates: 4386403..4387158 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JQ461_RS20400
• Protein ID: WP_003086329.1
• Coordinates: 4387293..4387661 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ461_RS20385 (JQ461_20365)	4381576..4384119	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
JQ461_RS20390 (JQ461_20370)	4384274..4385635	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
JQ461_RS20395 (JQ461_20375)	4386403..4387158	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
JQ461_RS20400 (JQ461_20380)	4387293..4387661	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JQ461_RS20405 (JQ461_20385)	4387734..4389020	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
JQ461_RS20410 (JQ461_20390)	4389076..4390569	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
JQ461_RS20415 (JQ461_20395)	4390744..4391220	+	477	WP_003112531.1	hypothetical protein	-
JQ461_RS20420 (JQ461_20400)	4391223..4391498	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T190166 WP_003112528.1 NZ_CP069324:c4387158-4386403 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190166 NZ_CP091125:181396-181671 [Serratia marcescens]
ATGCTCAACATCGAATGGACTCGAGTCGCACGTCAGGATCTGGGGGAGATTGTGGGATTTATCGCCAATGATAACCCTCT
GGCGGCCAGAAAAATGAAGGCTCTCATCATGGAGGCCGTCATTCCCGCCGCCAGACATCCTCGAATTTTCCGGCGCGGCA
GAGTGGACGGCACGCATGAAATCCCCCTGCATCCCCATTATCTCCTGGTTTACCAGGTGCTGGAAACTCACATCCGGGTG
TTGCGGGTTTTACATACGCGCCGACAATATCCTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190166 WP_003086329.1 NZ_CP069324:c4387661-4387293 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190166 NZ_CP091125:181199-181396 [Serratia marcescens]
ATGAGTATTTTACTGCTGCCTACGGTTGCCAAGATGGAAAATGAGGAGCGTACACAGGGCTATGAGCGCTGGTTTCTTGA
AAGAGTTCAGGAAGCCATGGATGAGCCACGGCCCGGTATTCCTCATGATGACGTGATGGCTGAAATGGATGCTGTTATCG
CTGCTGCAGAAGCCAGGATCAAAGCGAAGCAGGCATAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7