Detailed information of TA system TA190165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3407162..3408383
• Replicon: chromosome
• Accession: NZ_CP069324
• Organism: Pseudomonas aeruginosa strain R05

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JQ461_RS15755
• Protein ID: WP_003102740.1
• Coordinates: 3407805..3408383 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQ461_RS15750
• Protein ID: WP_003088112.1
• Coordinates: 3407162..3407716 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQ461_RS15725 (JQ461_15720)	3402730..3403281	+	552	WP_004343115.1	XRE family transcriptional regulator	-
JQ461_RS15730 (JQ461_15725)	3403278..3403676	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JQ461_RS15735 (JQ461_15730)	3403689..3404012	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
JQ461_RS15740 (JQ461_15735)	3404343..3404804	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JQ461_RS15745 (JQ461_15740)	3404809..3407004	+	2196	WP_010794059.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JQ461_RS15750 (JQ461_15745)	3407162..3407716	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JQ461_RS15755 (JQ461_15750)	3407805..3408383	+	579	WP_003102740.1	HD domain-containing protein	Toxin
JQ461_RS15760 (JQ461_15755)	3408492..3409679	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JQ461_RS15765 (JQ461_15760)	3409669..3411840	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JQ461_RS15770 (JQ461_15765)	3411830..3413107	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T190165 WP_003102740.1 NZ_CP069324:3407805-3408383 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T190165 NZ_CP091125:55443-55718 [Serratia marcescens]
ATGGCGTTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGT
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTTGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGACGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT190165 WP_003088112.1 NZ_CP069324:3407162-3407716 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT190165 NZ_CP091125:55183-55443 [Serratia marcescens]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAACAAGCCCTCGCCGCCTGGCTCGAGCAAGAGGAAGAGCGCCGTCGGTTAACGCTGGCGG
CGTTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAT
CCGCTGCCGGTACCGCGCTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07