Detailed information of TA system TA190155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 123713..124356
• Replicon: plasmid unnamed1
• Accession: NZ_CP069321
• Organism: Kosakonia cowanii strain Pa82

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JMT66_RS23845
• Protein ID: WP_232045605.1
• Coordinates: 123713..124129 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: JMT66_RS23850
• Protein ID: WP_232045608.1
• Coordinates: 124126..124356 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMT66_RS23810 (JMT66_23750)	118736..118972	-	237	WP_232045594.1	hypothetical protein	-
JMT66_RS23815 (JMT66_23755)	119476..119904	+	429	WP_232045597.1	GFA family protein	-
JMT66_RS23820 (JMT66_23760)	120013..120402	+	390	WP_074143445.1	GFA family protein	-
JMT66_RS23825 (JMT66_23765)	120602..121039	+	438	WP_232045598.1	VOC family protein	-
JMT66_RS23830 (JMT66_23770)	121325..121771	+	447	WP_232045600.1	DUF3290 domain-containing protein	-
JMT66_RS23835 (JMT66_23775)	121781..122416	+	636	WP_232045602.1	DUF421 domain-containing protein	-
JMT66_RS23840 (JMT66_23780)	122574..123554	-	981	WP_232045604.1	hypothetical protein	-
JMT66_RS23845 (JMT66_23785)	123713..124129	-	417	WP_232045605.1	type II toxin-antitoxin system VapC family toxin	Toxin
JMT66_RS23850 (JMT66_23790)	124126..124356	-	231	WP_232045608.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JMT66_RS23855 (JMT66_23795)	124410..124595	+	186	Protein_130	hypothetical protein	-
JMT66_RS23860 (JMT66_23800)	124893..125228	+	336	WP_200581166.1	hypothetical protein	-
JMT66_RS23865 (JMT66_23805)	125326..126027	+	702	WP_232045609.1	hypothetical protein	-
JMT66_RS23870 (JMT66_23810)	126024..126809	+	786	WP_232045610.1	site-specific integrase	-
JMT66_RS23875 (JMT66_23815)	127014..127262	+	249	WP_232045611.1	hypothetical protein	-
JMT66_RS23880 (JMT66_23820)	127318..127668	+	351	WP_232045770.1	hypothetical protein	-
JMT66_RS23885 (JMT66_23825)	127935..128429	-	495	WP_232045613.1	PBECR2 nuclease fold domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141409	141409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14957.21 Da
• Isoelectric Point: 7.2451

>T190155 WP_232045605.1 NZ_CP069321:c124129-123713 [Kosakonia cowanii]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVALVDAFCSRLDAVLPWDRA
AVDATTEIRVALRLAGTPIGTNDTAIAGHAIAAGAVLVTNNVREFERVPGLTLEDWAG

Nucleotide

• Length: 417 bp

>T190155 NZ_CP091123:511269-511619 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAACAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCAGGGCCTTCTCTTCGGCGGCCTTTTGTCGACGTGCTGAGAGGCTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATCGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8807.96 Da
• Isoelectric Point: 4.5112

>AT190155 WP_232045608.1 NZ_CP069321:c124356-124126 [Kosakonia cowanii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWRSFLELEKADPDFMAEREGVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT190155 NZ_CP091123:511623-511922 [Serratia marcescens]
ATGAGCACCCTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGTTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAATGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTTTAA