Detailed information of TA system TA190154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 93821..94731
• Replicon: plasmid unnamed1
• Accession: NZ_CP069321
• Organism: Kosakonia cowanii strain Pa82

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JMT66_RS23680
• Protein ID: WP_200134424.1
• Coordinates: 94261..94731 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JMT66_RS23675
• Protein ID: WP_094420625.1
• Coordinates: 93821..94264 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMT66_RS23665 (JMT66_23605)	91328..93106	+	1779	WP_232045557.1	sensor domain-containing phosphodiesterase	-
JMT66_RS23670 (JMT66_23610)	93423..93764	+	342	WP_232045558.1	hypothetical protein	-
JMT66_RS23675 (JMT66_23615)	93821..94264	+	444	WP_094420625.1	DUF2384 domain-containing protein	Antitoxin
JMT66_RS23680 (JMT66_23620)	94261..94731	+	471	WP_200134424.1	RES family NAD+ phosphorylase	Toxin
JMT66_RS23685 (JMT66_23625)	94822..95820	-	999	WP_232045562.1	tyrosine-type recombinase/integrase	-
JMT66_RS23690 (JMT66_23630)	96071..97279	-	1209	WP_232045563.1	diguanylate phosphodiesterase	-
JMT66_RS23695 (JMT66_23635)	97569..99155	+	1587	WP_232045567.1	methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141409	141409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17511.88 Da
• Isoelectric Point: 5.2138

>T190154 WP_200134424.1 NZ_CP069321:94261-94731 [Kosakonia cowanii]
MILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGTSMVYVSDVASLTMLETLVHLHASHIMDYFTLLRIDVPDEQIQKADMG
ELPGNWADEEAPSELAAYGDAWCFTGSSIALRVPSALSPVEFNYLLNPEHPEFSSVIKHAEEIPFRFDSRLKRARP

Nucleotide

• Length: 471 bp

>T190154 NZ_CP091123:447896-448171 [Serratia marcescens]
ATGCGTATCGAATGGGATAATGCAGCGCTACGGGACAGGGAACGTATTTTTGAGTTTCTTTATATTTTCAATCCACAGGC
GGCAGTGCAGGCTGATAACGAGATAGATAAAGCGGTAAAGCGTTTGCTGGATCATCCTGAGTTAGGTAAGCCTTGGTATG
GGAAGGCACGTAAGTTGCTAATTAACAAGGCCTCCCTGTTGCTGGTGTATATCATCGTCGATGATGTTATTAAGGTTTTC
TCGATTGCCCACCAGCGTGAGAAATTTCCAAACTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16459.90 Da
• Isoelectric Point: 9.8472

>AT190154 WP_094420625.1 NZ_CP069321:93821-94264 [Kosakonia cowanii]
MKTFNFSTIQARPPRLWQVAGLNTSDGVALVGQINDGLDGRVATVIADWAKMTQNDLRKMSGIPPTTYGRGLKARFSPDQ
SERLVRIIRVIDRAVDLFEGDKEEAQKWLKEPNRGLGWKVPAELMASETGAYEVMKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT190154 NZ_CP091123:447600-447887 [Serratia marcescens]
ATGGAAAGTCGCATCCAGTTTCGCATCGAAGATGAAACCAAGCGTTTGGCGCAAAAGGCCGCGGAAGCTAAAGGAACGAC
ATTGAGTGAAGCCTGCCGCCGCTTGGCGGAGCAAATGGCTGATGAGCAACGGGCCGCGGAGGAGCACGAAAATTGGTTGA
AAGATAAGGTGGATGCAGCTTTTTCCCGTCTGCATGAGGGTAATGCTGTCTACCTTAGCCAACAAGAAGTTGAAGAAAGC
ATGGATGCCTTTAAGGCAAAAATTAGAGCGAAATACGACCGAAAATAG