Detailed information of TA system TA190149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3652379..3652995
• Replicon: chromosome
• Accession: NZ_CP069319
• Organism: Kosakonia cowanii strain Pa82

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A807LE76
• Locus tag: JMT66_RS17485
• Protein ID: WP_023478287.1
• Coordinates: 3652777..3652995 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: JMT66_RS17480
• Protein ID: WP_042714473.1
• Coordinates: 3652379..3652753 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMT66_RS17470 (JMT66_17420)	3647476..3648675	+	1200	WP_042714476.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
JMT66_RS17475 (JMT66_17425)	3648698..3651847	+	3150	WP_094419552.1	multidrug efflux RND transporter permease subunit AcrB	-
JMT66_RS17480 (JMT66_17430)	3652379..3652753	+	375	WP_042714473.1	Hha toxicity modulator TomB	Antitoxin
JMT66_RS17485 (JMT66_17435)	3652777..3652995	+	219	WP_023478287.1	HHA domain-containing protein	Toxin
JMT66_RS17490 (JMT66_17440)	3653071..3653637	+	567	WP_200581037.1	maltose O-acetyltransferase	-
JMT66_RS17495 (JMT66_17445)	3653741..3654244	+	504	WP_232045057.1	YlaC family protein	-
JMT66_RS17500 (JMT66_17450)	3654365..3655066	+	702	WP_200581035.1	GNAT family protein	-
JMT66_RS17505 (JMT66_17455)	3655063..3655203	-	141	WP_023478279.1	type B 50S ribosomal protein L36	-
JMT66_RS17510 (JMT66_17460)	3655207..3655467	-	261	WP_124969359.1	type B 50S ribosomal protein L31	-
JMT66_RS17515 (JMT66_17465)	3655729..3656757	+	1029	WP_200581034.1	isopenicillin N synthase family oxygenase	-
JMT66_RS17520 (JMT66_17470)	3656780..3657586	+	807	WP_200581033.1	MetQ/NlpA family ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8657.04 Da
• Isoelectric Point: 7.9892

>T190149 WP_023478287.1 NZ_CP069319:3652777-3652995 [Kosakonia cowanii]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELTDDELTVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T190149 NZ_CP091122:c4379772-4379518 [Serratia marcescens]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAATGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14434.29 Da
• Isoelectric Point: 6.2831

>AT190149 WP_042714473.1 NZ_CP069319:3652379-3652753 [Kosakonia cowanii]
MDEYSPKRHDIAQLKFLCESLHQECLANLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKHDKDNKLIELIDEYL
DDTFMLFSSYGINTHDLQKWRKTGSKLFRSFVNVSKANPVSHSC

Nucleotide

• Length: 375 bp

>AT190149 NZ_CP091122:c4379521-4379177 [Serratia marcescens]
ATGATGAATAACACACTGAAAATTGACGGCCATACGGCCATCATCAACTTCGACCCAGAAATTGAAATGTTCCGGGGCGA
GTTTGTCGGGCTGAACGGTGGTGCTGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCG
CGGTCTTTCTCGATGAGTGCCAAAAAGACGGCATCGAGCCCTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCG
CCGGAACGCCATCAGGCGCTAGCCCTTGCCGCACAGGCCACCGGGCAGTCGATTAACGAGCTGCTGAATGAAGGCGTTGA
TTTAGTTATCGAAAAACACTCATGA

Structures

Toxin

• AlphaFold DB: A0A807LE76