Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 224451..225087 Replicon chromosome
Accession NZ_CP069296
Organism Metabacillus sp. cB07

Toxin (Protein)


Gene name mazF Uniprot ID A0A2N5FBL1
Locus tag JOJ89_RS01285 Protein ID WP_029286376.1
Coordinates 224737..225087 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A2N5FBR6
Locus tag JOJ89_RS01280 Protein ID WP_029286378.1
Coordinates 224451..224732 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JOJ89_RS01260 220770..221366 - 597 WP_203289360.1 rhomboid family intramembrane serine protease -
JOJ89_RS01265 221476..221841 + 366 WP_029286384.1 holo-ACP synthase -
JOJ89_RS01270 221983..222993 + 1011 WP_203289361.1 outer membrane lipoprotein carrier protein LolA -
JOJ89_RS01275 223122..224291 + 1170 WP_203289362.1 alanine racemase -
JOJ89_RS01280 224451..224732 + 282 WP_029286378.1 hypothetical protein Antitoxin
JOJ89_RS01285 224737..225087 + 351 WP_029286376.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JOJ89_RS01290 225227..226063 + 837 WP_029286374.1 STAS domain-containing protein -
JOJ89_RS01295 226060..226425 + 366 WP_029286372.1 STAS domain-containing protein -
JOJ89_RS01300 226428..226829 + 402 WP_029286370.1 anti-sigma regulatory factor -
JOJ89_RS01305 226841..227848 + 1008 WP_203289363.1 PP2C family protein-serine/threonine phosphatase -
JOJ89_RS01310 227912..228241 + 330 WP_029286366.1 anti-sigma factor antagonist -
JOJ89_RS01315 228238..228720 + 483 WP_029286364.1 anti-sigma B factor RsbW -
JOJ89_RS01320 228686..229474 + 789 WP_029286362.1 RNA polymerase sigma factor SigB -
JOJ89_RS01325 229474..230073 + 600 WP_029566340.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13036.06 Da        Isoelectric Point: 4.8781

>T190115 WP_029286376.1 NZ_CP069296:224737-225087 [Metabacillus sp. cB07]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDSKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T190115 NZ_CP091099:1433357-1433692 [Pantoea agglomerans]
ATGGTAAGCCGTTTTGTCCCTGATGCGGGAGATATCATCTGGGTGGATTTTGATCCGACCAAAGGCAGTGAGCAGGCAGG
TCACAGACCCGCTATTGTTCTTAGCCCGTTTGCTTACAATAACAAGACCGGCATGTGTCTCTGTGTCCCCTGCACCACCC
TGTCAAAGGGCTATCCGTTTGAAGTCCCCATAAGTGCTGAAAAAGAAAGCGTCGCCTTATCCGATCAAGTGAAAAGCATC
GCCTGGAAAGTCAGGGGAGCAACCAAAAAAGGCGTTGTTTCCGCAAACGAACTTCAGCAAATTCGCGCTAAGATAAAAGT
TATCATTGGCTTATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10572.01 Da        Isoelectric Point: 7.0156

>AT190115 WP_029286378.1 NZ_CP069296:224451-224732 [Metabacillus sp. cB07]
VSESSATTEILIRLPQALVSELDGLVKQENGNRSELIYQATKMYIRERKKRQIRESMRRGYMEMAKINLNIASEAFQAES
EADHTVERLVSGG

Download         Length: 282 bp

>AT190115 NZ_CP091099:1433108-1433356 [Pantoea agglomerans]
ATGATTCATGGAAATGTTAAACGCTGGGGTAACTCTCCGGCGATACGTATCCCGGCGACATTGATGCAGGCACTAAATCT
TAATATTGATGACGAAATTAAAATCGATTTAGTTGAAGGGAAACTCATTATCGAGCCTGTCAAACAGGAAGTGGGTTACT
CTCTTGAAGAACTGCTTGAAGGCGTGACAGAGGAAAATCTGCACGATCCAGTCGAATGGGGTGAACCTGTCGGGAAGGAG
ATTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N5FBL1

Antitoxin

Source ID Structure
AlphaFold DB A0A2N5FBR6

References