Detailed information of TA system TA190114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200714..201350
• Replicon: chromosome
• Accession: NZ_CP069288
• Organism: Priestia megaterium strain ATCC 14581

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: H8R29_RS01135
• Protein ID: WP_013055004.1
• Coordinates: 201000..201350 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: H8R29_RS01130
• Protein ID: WP_013055003.1
• Coordinates: 200714..200995 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8R29_RS01105	196080..197051	+	972	WP_034654859.1	UV DNA damage repair endonuclease UvsE	-
H8R29_RS01110	197060..197662	-	603	WP_034654858.1	rhomboid family intramembrane serine protease	-
H8R29_RS01115	197727..198092	+	366	WP_025753519.1	holo-ACP synthase	-
H8R29_RS01120	198151..199209	+	1059	WP_016762756.1	outer membrane lipoprotein carrier protein LolA	-
H8R29_RS01125	199323..200513	+	1191	WP_034654856.1	alanine racemase	-
H8R29_RS01130	200714..200995	+	282	WP_013055003.1	hypothetical protein	Antitoxin
H8R29_RS01135	201000..201350	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
H8R29_RS01140	201510..202346	+	837	WP_034654854.1	STAS domain-containing protein	-
H8R29_RS01145	202349..202705	+	357	WP_013055006.1	STAS domain-containing protein	-
H8R29_RS01150	202709..203110	+	402	WP_013055007.1	anti-sigma regulatory factor	-
H8R29_RS01155	203124..204134	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
H8R29_RS01160	204194..204526	+	333	WP_013055009.1	anti-sigma factor antagonist	-
H8R29_RS01165	204523..205008	+	486	WP_016762758.1	anti-sigma B factor RsbW	-
H8R29_RS01170	204974..205768	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T190114 WP_013055004.1 NZ_CP069288:201000-201350 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T190114 NZ_CP091099:1170579-1171892 [Pantoea agglomerans]
ATGCAAAGGCTGATTGCGTGGATGAACGGCGAGCGGGTAGGCGTACTCACCCGGCAGCGAAACGGCGCTCATGAGTTTCA
GTATGATACCGGCTGGCTGAGTAATCCGCTCTCCAGGCCGCTGTCACTTTCGATGCCGTTGCAGGTTAAGGCTCTCACCT
CACACGCCGTGATTAACTTCTTCGATAATCTGCTGCCGGATAGCCCATTGATTCGCGACAGAATCGTGGCGCGTTACCAG
ATTAAATCCCGTCAGCCGTTTGATCTGTTGCAGGAAGTGGGACGTGACAGCGTCGGGGCAACAATGCTGCTGCCGCCCGA
TATGCCACTGCCCGCACTGCAGGCCCGCTATGAAGTGCTGGATGAAACCCGGCTCGAAAGCGTGCTGTCGGCCTATCAGT
CAGATATCCCGCTGGGGATGCTGCGTGAAGAGACCGATTTCCGTATCTCGGTCGCGGGGGCGCAGGAGAAAACCGCACTG
CTGCTAACCGATGAAGGCTGGAGCATTCCTGCCGGTGCCACGCCCACCTCGCACATCATTAAGCTGCCGATCGGAGAGAT
TAAACAGCCGGCCGCGACGCTGGATATGCGTGAAAGCGTGGAGAATGAGTATCTCTGTCTGGCGCTGGCCCGTGCACTCG
GTATCGACGTGCCGCAGGCAACGATTATCCGTGCCGGACAGATGCGCGCCCTGGCGGTAGAACGTTTTGACCGCCGCTGG
TCACGCGATAAAACCCGGTTGATTCGCCTGCCGCAGGAGGATTTCTGTCAGGCGCTGGGACTGCCATCTTCAACCAAATA
TGAGTCGGATGGCGGGCCGGGTATTGCGGAGATCATGCAGATACTGATGGGATCGAGCAACGCCATCGAAGATCGCTATC
GCTTTATGATGTTCCAGGTGTTTCAGTGGCTGATTGGCGCAACCGACGGTCACGCCAAGAACTTCTCACTGTTTATTGAG
CGCGGCGGCAGTTATCGACTGACTCCCTTTTATGATGTGATCTCCGCTTATCCGGTACTGGGCGGCACCGGTCTGAACAA
GCGAGCGCTGAAACTGGCGATGGGTCTGCGTGCCACTAAGGGGAAGAAGACAGAGATCGACAAAATCTTCCCGCGCCATT
TTATCGCCACGGCAAAAAGCGTCAGTTTTGATCGTGACAGTTTGCAGCAGATCTTCGACTTCTTTATTAAGGCCTTTCCC
CAGGCAGTGGTCTCGGTGCGCGATGCCTTGCCGCCCGATTTCCCGCAGCACATCGCCGACGCCATTTTTAGTCATTCACT
GATTATGCTGGAGCGGCTGCGGCACAAAGAGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT190114 WP_013055003.1 NZ_CP069288:200714-200995 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT190114 NZ_CP091099:1170331-1170579 [Pantoea agglomerans]
ATGATATACAGCCCGCTACAGCTGGCAAACCGGCTAAAGCTCATTCGTCAGCGCAATGGCTGGACCCAGAGTGAGCTGGC
GAAAAAGGTCGGCCTGAAACAGGCCACCATCTCTCACTTTGAAAATGCCCCTGACAGTACCTCGCTGGCAACGCTGTTTA
AGCTGTTACAGTCGCTGGAGCTGAGTCTGGAAGTTCAGGAGAAAGAGGAGGCCGGTGCGCCTCAGCTGCATGATGAGGAT
GAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9