Detailed information of TA system TA190113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 504181..504770
• Replicon: chromosome
• Accession: NZ_CP069278
• Organism: Bifidobacterium longum strain HN001

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: I3AVV6
• Locus tag: JQN89_RS02465
• Protein ID: WP_007057518.1
• Coordinates: 504181..504489 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I3AVV5
• Locus tag: JQN89_RS02470
• Protein ID: WP_007057517.1
• Coordinates: 504486..504770 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQN89_RS02450	499927..503046	-	3120	WP_007057919.1	alpha-mannosidase	-
JQN89_RS02455	503207..503695	-	489	WP_032684949.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JQN89_RS02460	503854..504159	+	306	WP_049137035.1	hypothetical protein	-
JQN89_RS02465	504181..504489	+	309	WP_007057518.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JQN89_RS02470	504486..504770	+	285	WP_007057517.1	putative addiction module antidote protein	Antitoxin
JQN89_RS02480	504978..505199	+	222	WP_077422270.1	holin	-
JQN89_RS02490	506040..506969	-	930	WP_053495500.1	FtsQ-type POTRA domain-containing protein	-
JQN89_RS02495	507104..508642	-	1539	WP_007054402.1	UDP-N-acetylmuramate--L-alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-94)

Antitoxin

• Uncharacterized protein HI1420 (3-92)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11638.44 Da
• Isoelectric Point: 10.3171

>T190113 WP_007057518.1 NZ_CP069278:504181-504489 [Bifidobacterium longum]
MEIKQSAEYRKWFKKLRDHKAKAAIQARLDVCKLAGRPFGDIKPVGGPVSEMRFHTGAGYRVYFAMQGNVLMLLLAGGDK
STQQTDIRQAHDILNDYKEQRQ

Nucleotide

• Length: 309 bp

>T190113 NZ_CP091099:c845770-845453 [Pantoea agglomerans]
GTGGCGACGTTAAGGAATATTGAAAGTTTCAGGGATGACTGGCTTAAGGATTATTTTTTATATGGCAAATTTTCTAAAAG
AATACCGTCAAGCCTCTCATCAGCGCTGGCGCGAAAGCTCGACATGATCAATGCGGCGATTAACTATAAGGATTTAAGGT
CGCCGCCTGGCAATCGTTATGAGGAACTAAATCCGCCTCTCAAGGGATATGCTTCAATCAGGGTCAATGAGCAATATCGG
CTGATCTTTAAATGGATAGAAGGTAAGGCCGTTGACCTTTATCTGGATGCTCATAGTTATAAAACGCACAAGCGATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10158.37 Da
• Isoelectric Point: 4.3707

>AT190113 WP_007057517.1 NZ_CP069278:504486-504770 [Bifidobacterium longum]
MSTEFTDYDTSEYLENEQDIIAYLNAIAEYDDPALMQAALGNVAKARGMTQIAKDAGVGRESLYKSLSKDGNPSFQTIAK
VIHALGGRLTIQAA

Nucleotide

• Length: 285 bp

>AT190113 NZ_CP091099:c845372-845040 [Pantoea agglomerans]
ATGAACCAGGCGACCCGCAAACCGACCACTGTCGGTGACGTATTACTTTACGAATATCTGGAGCCAACCGGCCTGAAGAT
TCTCGACTTAGCGGAGATGCTCAAAGTGCACCGCAACACCGTCAGCGCTCTGGTCAACAATAACCGGAAGCTGACGCCGG
ATATGGCTTTCAGGCTGGCTGCAGCTTTCGAAACCAGCGTTGAGTTCTGGCTTAATCTGCAAAACAGTGTCGATATCTGG
GAAGTGATGCATGATGCCAAGGCACAGGAGGAGATCAGTCGCGTAACACCGTTGAAGGATTTTCTGGTGCAGAAAGAGAA
TCAGCTGGCGTGA

Structures

Toxin

• AlphaFold DB: I3AVV6

Antitoxin

• AlphaFold DB: I3AVV5