Detailed information of TA system TA190109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2880242..2880903
• Replicon: chromosome
• Accession: NZ_CP069245
• Organism: Ralstonia pseudosolanacearum strain PeaFJ1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E7Z845
• Locus tag: JNO62_RS13205
• Protein ID: WP_016727753.1
• Coordinates: 2880490..2880903 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7Z6P3
• Locus tag: JNO62_RS13200
• Protein ID: WP_019717749.1
• Coordinates: 2880242..2880493 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNO62_RS13190 (JNO62_13105)	2878071..2879285	+	1215	WP_162905114.1	hypothetical protein	-
JNO62_RS13195	2879467..2880162	-	696	Protein_2579	DUF6441 family protein	-
JNO62_RS13200 (JNO62_13120)	2880242..2880493	+	252	WP_019717749.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JNO62_RS13205 (JNO62_13125)	2880490..2880903	+	414	WP_016727753.1	type II toxin-antitoxin system VapC family toxin	Toxin
JNO62_RS13210	2881185..2881430	+	246	WP_249902484.1	hypothetical protein	-
JNO62_RS13215	2881419..2881997	-	579	WP_249902485.1	DNA cytosine methyltransferase	-
JNO62_RS13220 (JNO62_13135)	2882621..2882911	+	291	WP_016723655.1	H-NS family nucleoid-associated regulatory protein	-
JNO62_RS13225 (JNO62_13140)	2882954..2883598	-	645	WP_058908389.1	DUF6441 family protein	-
JNO62_RS13230 (JNO62_13145)	2883597..2883770	+	174	WP_249902486.1	hypothetical protein	-
JNO62_RS13235 (JNO62_13150)	2883805..2884194	-	390	WP_111375425.1	hypothetical protein	-
JNO62_RS13240 (JNO62_13155)	2884203..2884976	-	774	WP_089190857.1	hypothetical protein	-
JNO62_RS13245 (JNO62_13160)	2885077..2885523	-	447	WP_089190858.1	hypothetical protein	-
JNO62_RS13250 (JNO62_13165)	2885529..2885831	-	303	WP_089190859.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2858970..2898065	39095

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15586.89 Da
• Isoelectric Point: 6.2337

>T190109 WP_016727753.1 NZ_CP069245:2880490-2880903 [Ralstonia pseudosolanacearum]
MSYLIDTNVLSELRRKAPDARVVAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDVQLINPWEA

Nucleotide

• Length: 414 bp

>T190109 NZ_CP091098:c46278-46027 [Pantoea agglomerans]
ATGAGAGCAATCAGTTACAGCGAAGCCCGACAGAATCTGTCTGCCACTATGGTTCAGACAGTTGAAGATAAAGCGCCGGT
ATTGATCACTCGCCAGAACGGTGAATCCTGCGTGCTGATGTCGCTTGAGGAATATAACTCACTGGAAGAGACGGCATACC
TGCTGCGGTCACCTAAAAATGCGCGCAGGTTGATGGATTCTATCGAAAGCCTGCAAAGCGGTAACGGGACTGACAGGGAT
ATTATTGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9305.49 Da
• Isoelectric Point: 5.0774

>AT190109 WP_019717749.1 NZ_CP069245:2880242-2880493 [Ralstonia pseudosolanacearum]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT190109 NZ_CP091098:c46030-45776 [Pantoea agglomerans]
GTGAAACTAACCTGGTCCGCAGAAGCCTGGGAAGATTATCTCTACTGGCAGGATGCCGATAAAAAAACCGTTAAAAAGAT
AAACGAGCTGCTCAAAGATGCCAGCAGGACGCCTTTTGAAGGCAAAGGAAAACCCGAGCCGTTAAAGCATAATCTTGCAG
GTTTCTGGTCACGCAGAATCACGGCAGAACATCGGCTGGTGTACGCTGTCAGCGGGGATGCTCTATTGATTGCCTCATGC
CGTTATCATTACTGA