Detailed information of TA system TA190108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2036542..2038128
• Replicon: chromosome
• Accession: NZ_CP069245
• Organism: Ralstonia pseudosolanacearum strain PeaFJ1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9E8CN90
• Locus tag: JNO62_RS09215
• Protein ID: WP_064047702.1
• Coordinates: 2036791..2038128 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A223GLT8
• Locus tag: JNO62_RS09210
• Protein ID: WP_028853251.1
• Coordinates: 2036542..2036787 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNO62_RS09180 (JNO62_09110)	2032605..2033531	-	927	WP_071013782.1	muramoyltetrapeptide carboxypeptidase	-
JNO62_RS09185 (JNO62_09115)	2033528..2034079	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
JNO62_RS09190 (JNO62_09120)	2034099..2034575	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
JNO62_RS09195 (JNO62_09125)	2034643..2035281	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
JNO62_RS09200	2035453..2035575	+	123	Protein_1805	2-hydroxychromene-2-carboxylate isomerase	-
JNO62_RS09205 (JNO62_09130)	2035599..2036438	+	840	WP_016725278.1	oxaloacetate decarboxylase	-
JNO62_RS09210 (JNO62_09135)	2036542..2036787	+	246	WP_028853251.1	helix-turn-helix transcriptional regulator	Antitoxin
JNO62_RS09215 (JNO62_09140)	2036791..2038128	+	1338	WP_064047702.1	type II toxin-antitoxin system HipA family toxin	Toxin
JNO62_RS09220 (JNO62_09145)	2038125..2039327	+	1203	WP_071013776.1	MFS transporter	-
JNO62_RS09225 (JNO62_09150)	2039591..2040319	+	729	WP_011001393.1	DsbA family protein	-
JNO62_RS09230 (JNO62_09155)	2040332..2040928	-	597	WP_016727240.1	RNA 2',3'-cyclic phosphodiesterase	-
JNO62_RS09235 (JNO62_09160)	2041076..2041774	-	699	WP_058908491.1	protocatechuate 3,4-dioxygenase subunit alpha	-
JNO62_RS09240 (JNO62_09165)	2041771..2042511	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-63)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48768.81 Da
• Isoelectric Point: 6.7232

>T190108 WP_064047702.1 NZ_CP069245:2036791-2038128 [Ralstonia pseudosolanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T190108 NZ_CP091098:c46030-45776 [Pantoea agglomerans]
GTGAAACTAACCTGGTCCGCAGAAGCCTGGGAAGATTATCTCTACTGGCAGGATGCCGATAAAAAAACCGTTAAAAAGAT
AAACGAGCTGCTCAAAGATGCCAGCAGGACGCCTTTTGAAGGCAAAGGAAAACCCGAGCCGTTAAAGCATAATCTTGCAG
GTTTCTGGTCACGCAGAATCACGGCAGAACATCGGCTGGTGTACGCTGTCAGCGGGGATGCTCTATTGATTGCCTCATGC
CGTTATCATTACTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8482.80 Da
• Isoelectric Point: 10.4693

>AT190108 WP_028853251.1 NZ_CP069245:2036542-2036787 [Ralstonia pseudosolanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Nucleotide

• Length: 246 bp

>AT190108 NZ_CP091098:c46278-46027 [Pantoea agglomerans]
ATGAGAGCAATCAGTTACAGCGAAGCCCGACAGAATCTGTCTGCCACTATGGTTCAGACAGTTGAAGATAAAGCGCCGGT
ATTGATCACTCGCCAGAACGGTGAATCCTGCGTGCTGATGTCGCTTGAGGAATATAACTCACTGGAAGAGACGGCATACC
TGCTGCGGTCACCTAAAAATGCGCGCAGGTTGATGGATTCTATCGAAAGCCTGCAAAGCGGTAACGGGACTGACAGGGAT
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A223GLT8