Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 362039..362672 Replicon chromosome
Accession NZ_CP069245
Organism Ralstonia pseudosolanacearum strain PeaFJ1

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag JNO62_RS01650 Protein ID WP_016725172.1
Coordinates 362259..362672 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag JNO62_RS01645 Protein ID WP_011002949.1
Coordinates 362039..362272 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNO62_RS01630 (JNO62_01625) 357627..358922 - 1296 WP_011002952.1 MFS transporter -
JNO62_RS01635 (JNO62_01630) 358930..360162 - 1233 WP_064478113.1 AGE family epimerase/isomerase -
JNO62_RS01640 (JNO62_01635) 360375..361982 + 1608 WP_020832850.1 glucan biosynthesis protein -
JNO62_RS01645 (JNO62_01640) 362039..362272 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JNO62_RS01650 (JNO62_01645) 362259..362672 + 414 WP_016725172.1 PIN domain-containing protein Toxin
JNO62_RS01655 (JNO62_01650) 362647..363273 - 627 WP_019718918.1 DUF1415 family protein -
JNO62_RS01660 (JNO62_01655) 363464..363835 + 372 WP_071015009.1 hypothetical protein -
JNO62_RS01665 (JNO62_01660) 363897..365393 - 1497 WP_011002945.1 glycerol kinase GlpK -
JNO62_RS01670 (JNO62_01665) 365555..367291 - 1737 WP_019718916.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T190107 WP_016725172.1 NZ_CP069245:362259-362672 [Ralstonia pseudosolanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T190107 NZ_CP091097:150032-150502 [Pantoea agglomerans]
ATGGAAGACAGCACGGTTATAAATGGATGGCACGTTTATGTTCCTCCCTGTTTTGAATCGCAACTGATTGCGCTGACCAA
TGAAGTGGAGTTACTCAGGGCTAAATATCCTGAAAGTTACCAGCGAAAAGCGACGACGAAGCTTTTAGCCGCTGTGTTTA
AAGTTATCAATAGTGAGATTTGCGCCGATCCACATCAGGCAAAGTTTCGCCAGGGCGATACGCTGGGAGAACAAAATAAG
CATTGGTTCCGTGCAAAGTTTTTGATGCAGTATCGTTTGTTCTTTAGATTCAGCGAGCAACATAAAACCATCATTCTTGC
ATGGATGAATGACGCTGAGACCAAACGTGCTTATGGCAGTAAGCGTGACGCCTATAAAGTATTTTCAGGAATGTTGCATA
ACGGCTATCCACCTGACGACTGGGCATTGCTGTTAAAGCAATCTCAGGATTGGGCGAATACAAATTCTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT190107 WP_011002949.1 NZ_CP069245:362039-362272 [Ralstonia pseudosolanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT190107 NZ_CP091097:149681-150025 [Pantoea agglomerans]
ATGGGAACTCTGACTCATGCAAACATCTCTCTGCGTGCTTCATCAAAACTGACAGCCCGCGGACAGACCACCATCCCTGC
CGCAGTTCGTGATGCGATGAATCTGCAACCTGGTGAGAATATCGAATACGCTGTTCTGCCGGGCGGCAAAGTCCTTATTA
CCCGTCAGGAAACGGTGGCTGATGATGCTCCAGTGAAGGCGAGCTTTCTGAGTTTTCTTCCTGCTGACAGGCGCAACACG
GCTGCAAATATTAAAATGCTGGATCGAGGATTATTAACTCGCATTACATCGTTGACCGAAGGTATGGACGTTGATTTGGA
TGCTCCACTCATGGACGATGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References