Detailed information of TA system TA190097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1230296..1231213
• Replicon: chromosome
• Accession: NZ_CP069214
• Organism: Bacillus velezensis strain GUAL210

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: JOF24_RS06425
• Protein ID: WP_007407256.1
• Coordinates: 1230467..1231213 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JOF24_RS06420
• Protein ID: WP_003154807.1
• Coordinates: 1230296..1230466 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JOF24_RS06385	1225519..1227150	+	1632	WP_015417294.1	pyocin knob domain-containing protein	-
JOF24_RS06390	1227163..1227534	+	372	WP_015417295.1	YomQ/XkdW protein, phage-like element PBSX	-
JOF24_RS06395	1227539..1227736	+	198	WP_012117366.1	XkdX family protein	-
JOF24_RS06400	1227793..1228554	+	762	WP_031378811.1	hypothetical protein	-
JOF24_RS06405	1228606..1228869	+	264	WP_031378810.1	hemolysin XhlA family protein	-
JOF24_RS06410	1228883..1229146	+	264	WP_003154813.1	phage holin	-
JOF24_RS06415	1229160..1230038	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
JOF24_RS06420	1230296..1230466	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JOF24_RS06425	1230467..1231213	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JOF24_RS06430	1231317..1232315	-	999	WP_003154805.1	inorganic phosphate transporter	-
JOF24_RS06435	1232328..1232945	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JOF24_RS06440	1233231..1234547	-	1317	WP_007610842.1	amino acid permease	-
JOF24_RS06445	1234870..1235820	+	951	WP_033575306.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T190097 WP_007407256.1 NZ_CP069214:c1231213-1230467 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T190097 NZ_CP091081:c2981219-2980698 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGTGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTAGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTGGCATTGCAGGCGGTGGGCGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGATGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT190097 WP_003154807.1 NZ_CP069214:c1230466-1230296 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT190097 NZ_CP091081:c2981495-2981226 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCACCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14