Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 490504..491141 Replicon chromosome
Accession NZ_CP069214
Organism Bacillus velezensis strain GUAL210

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JOF24_RS02465 Protein ID WP_003156187.1
Coordinates 490791..491141 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag JOF24_RS02460 Protein ID WP_003156188.1
Coordinates 490504..490785 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JOF24_RS02440 486869..487468 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
JOF24_RS02445 487561..487926 + 366 WP_007410229.1 holo-ACP synthase -
JOF24_RS02450 488091..489098 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
JOF24_RS02455 489215..490384 + 1170 WP_061890435.1 alanine racemase -
JOF24_RS02460 490504..490785 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JOF24_RS02465 490791..491141 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JOF24_RS02470 491259..492080 + 822 WP_033575045.1 STAS domain-containing protein -
JOF24_RS02475 492085..492450 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
JOF24_RS02480 492453..492854 + 402 WP_003156178.1 anti-sigma regulatory factor -
JOF24_RS02485 492866..493873 + 1008 WP_015416875.1 PP2C family protein-serine/threonine phosphatase -
JOF24_RS02490 493937..494266 + 330 WP_033575044.1 anti-sigma factor antagonist -
JOF24_RS02495 494263..494745 + 483 WP_007609591.1 anti-sigma B factor RsbW -
JOF24_RS02500 494711..495499 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
JOF24_RS02505 495499..496101 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T190096 WP_003156187.1 NZ_CP069214:490791-491141 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T190096 NZ_CP091081:1977165-1977268 [Enterobacter roggenkampii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT190096 WP_003156188.1 NZ_CP069214:490504-490785 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT190096 NZ_CP091081:c1977273-1977130 [Enterobacter roggenkampii]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References