Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 123161..123684 | Replicon | plasmid pSP968_1 |
| Accession | NZ_CP069204 | ||
| Organism | Klebsiella pneumoniae strain SP968 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | V5RNQ6 |
| Locus tag | IEE96_RS26780 | Protein ID | WP_015632464.1 |
| Coordinates | 123161..123466 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | V5RQR8 |
| Locus tag | IEE96_RS26785 | Protein ID | WP_015632465.1 |
| Coordinates | 123466..123684 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IEE96_RS26735 (IEE96_26650) | 118191..118619 | - | 429 | WP_015632456.1 | plasmid partitioning/stability family protein | - |
| IEE96_RS26740 (IEE96_26655) | 118612..119592 | - | 981 | WP_015632457.1 | plasmid segregation protein ParM | - |
| IEE96_RS26745 (IEE96_26660) | 119953..120135 | + | 183 | WP_015632458.1 | type II toxin-antitoxin system HicA family toxin | - |
| IEE96_RS26750 (IEE96_26665) | 120161..120604 | + | 444 | WP_015632459.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| IEE96_RS26755 (IEE96_26670) | 120810..120932 | - | 123 | Protein_137 | transcriptional regulator | - |
| IEE96_RS26760 (IEE96_26675) | 120946..121173 | - | 228 | WP_016162072.1 | hypothetical protein | - |
| IEE96_RS26765 | 121405..121860 | + | 456 | WP_015632461.1 | helix-turn-helix domain-containing protein | - |
| IEE96_RS26770 | 122112..122207 | - | 96 | Protein_140 | IS1 family transposase | - |
| IEE96_RS26775 (IEE96_26690) | 122672..123049 | - | 378 | WP_016162069.1 | hypothetical protein | - |
| IEE96_RS26780 (IEE96_26695) | 123161..123466 | - | 306 | WP_015632464.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| IEE96_RS26785 (IEE96_26700) | 123466..123684 | - | 219 | WP_015632465.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| IEE96_RS26790 (IEE96_26705) | 123870..124895 | + | 1026 | WP_015632466.1 | IS110 family transposase | - |
| IEE96_RS26795 (IEE96_26710) | 125190..125675 | + | 486 | Protein_145 | IS5 family transposase | - |
| IEE96_RS26800 (IEE96_26715) | 125839..126270 | + | 432 | WP_015632467.1 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| IEE96_RS26805 (IEE96_26720) | 126270..127541 | + | 1272 | WP_016162068.1 | Y-family DNA polymerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | qnrB1 / dfrA14 / ARR-3 / rmtF | - | 1..127934 | 127934 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11361.90 Da Isoelectric Point: 5.5252
>T190092 WP_015632464.1 NZ_CP069204:c123466-123161 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI
Download Length: 306 bp
>T190092 NZ_CP091081:46003-46401 [Enterobacter roggenkampii]
ATGAGACATATGCTGGATACGAATATTGTGAGTCACTTGTTTAAACGACACCCGGAGGTTGTAAGCAGAATGACATGTCT
GGCGCCGGGTGACGTTTGTATCTCAAGCATTACCGAAGCGGAATTGCTGTATGGTGCTGACAAGAAGAAAAGCAGTAGAT
TGAAAGAAACAATCCGGGAGTTTCTCAATACCATCAAGATTTGCGACTGGGACAGCGACGCCGCAGCAACGTACGGTGAA
CTCCGTGCAACCCTGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAACTGATCGCTGCACATGCTATCAGTCGGGG
AACAACGATCGTTACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAACGTTGTGA
ATGAGACATATGCTGGATACGAATATTGTGAGTCACTTGTTTAAACGACACCCGGAGGTTGTAAGCAGAATGACATGTCT
GGCGCCGGGTGACGTTTGTATCTCAAGCATTACCGAAGCGGAATTGCTGTATGGTGCTGACAAGAAGAAAAGCAGTAGAT
TGAAAGAAACAATCCGGGAGTTTCTCAATACCATCAAGATTTGCGACTGGGACAGCGACGCCGCAGCAACGTACGGTGAA
CTCCGTGCAACCCTGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAACTGATCGCTGCACATGCTATCAGTCGGGG
AACAACGATCGTTACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAACGTTGTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8194.13 Da Isoelectric Point: 6.2224
>AT190092 WP_015632465.1 NZ_CP069204:c123684-123466 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW
Download Length: 219 bp
>AT190092 NZ_CP091081:45740-46006 [Enterobacter roggenkampii]
ATGGAACGCACAGCAAAACTATTCAGAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGCGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCATGA
ATGGAACGCACAGCAAAACTATTCAGAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGCGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6C0ND19 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6C0L1T7 |