Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5270589..5271214 Replicon chromosome
Accession NZ_CP069203
Organism Klebsiella pneumoniae strain SP968

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IEE96_RS25640 Protein ID WP_002882817.1
Coordinates 5270589..5270972 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IEE96_RS25645 Protein ID WP_004150355.1
Coordinates 5270972..5271214 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEE96_RS25625 (5267955) 5267955..5268857 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IEE96_RS25630 (5268854) 5268854..5269489 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IEE96_RS25635 (5269486) 5269486..5270415 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IEE96_RS25640 (5270589) 5270589..5270972 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IEE96_RS25645 (5270972) 5270972..5271214 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
IEE96_RS25650 (5271419) 5271419..5272336 + 918 WP_021466676.1 alpha/beta hydrolase -
IEE96_RS25655 (5272350) 5272350..5273291 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IEE96_RS25660 (5273336) 5273336..5273773 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
IEE96_RS25665 (5273770) 5273770..5274630 - 861 WP_004146232.1 virulence factor BrkB family protein -
IEE96_RS25670 (5274624) 5274624..5275223 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T190091 WP_002882817.1 NZ_CP069203:c5270972-5270589 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T190091 NZ_CP091080:14546-14872 [Xanthomonas arboricola pv. pruni]
ATGATGCGCGGCGACCTTGTGACCATCGCCATGCAGGGCGACTTTGGCAAGCCACGGCCTGCGCTTGTGGTTCAGGCCAA
CCAGTTCAGCGAGCACGGCAGCGTTACCGTGCTGCCTGTCACCAGCACACTTAATGCCGCGCCGTTGCTGCGCGTCACGG
TTCAGCCGAGCGCAGAGAACGGTTTGCAGAAGCCATCGCAGGTGATGGTCGACAAGGCGATGACGGTGCTGCGCAGCAAG
GTAGGGCCAGCTTTCGGGCGCATCGACGCGGACGCACTGCTTGAGGTTGAGCGTTGCTTGGCCGTGTTCTTGGGGATTGC
GAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT190091 WP_004150355.1 NZ_CP069203:c5271214-5270972 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT190091 NZ_CP091080:14325-14549 [Xanthomonas arboricola pv. pruni]
ATGGCAACAGCACACGTTAATCAGCGGGTTCAGAAGCATCGCGATGCATTACGCATGGCGGGGCTTCGTCCGGTTCAAAT
CTGGGTGCCGGATACGCGCCGCCCCGACTTCGCGGAGGAGTGCCGCCGCCAATGCCTGCTTGTGGCTCAGGCCGACAGCG
CCGACACCGCCATGCAGCAGTTCATGGATGAAGCCTTGGCAGACGTGGACGGCTGGACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References