Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1495254..1495897 Replicon chromosome
Accession NZ_CP069203
Organism Klebsiella pneumoniae strain SP968

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag IEE96_RS07435 Protein ID WP_032433387.1
Coordinates 1495481..1495897 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag IEE96_RS07430 Protein ID WP_001261276.1
Coordinates 1495254..1495484 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEE96_RS07415 (1490276) 1490276..1491363 + 1088 Protein_1450 transcriptional repressor PifC -
IEE96_RS07420 (1491366) 1491366..1493606 + 2241 WP_045326788.1 P-loop NTPase fold protein -
IEE96_RS07425 (1494134) 1494134..1494949 - 816 WP_032433388.1 hypothetical protein -
IEE96_RS07430 (1495254) 1495254..1495484 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IEE96_RS07435 (1495481) 1495481..1495897 + 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
IEE96_RS07440 (1496053) 1496053..1497033 + 981 WP_032433385.1 hypothetical protein -
IEE96_RS07445 (1497228) 1497228..1498799 - 1572 WP_032433383.1 AAA family ATPase -
IEE96_RS07450 (1499118) 1499118..1499366 + 249 WP_032433382.1 hypothetical protein -
IEE96_RS07455 (1499425) 1499425..1499943 + 519 WP_045326794.1 hypothetical protein -
IEE96_RS07460 (1499974) 1499974..1500465 + 492 WP_032433378.1 hypothetical protein -
IEE96_RS07465 (1500525) 1500525..1500728 + 204 WP_032433376.1 HHA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1482168..1523624 41456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T190076 WP_032433387.1 NZ_CP069203:1495481-1495897 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T190076 NZ_CP091069:c61994-61683 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT190076 WP_001261276.1 NZ_CP069203:1495254-1495484 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT190076 NZ_CP091069:c61686-61267 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References