Detailed information of TA system TA190069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 3403304..3404812
• Replicon: chromosome
• Accession: NZ_CP069198
• Organism: Pseudomonas aeruginosa strain 152962

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JQM61_RS15940
• Protein ID: WP_012613851.1
• Coordinates: 3404225..3404812 (-)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JQM61_RS15935
• Protein ID: WP_012613852.1
• Coordinates: 3403304..3404158 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQM61_RS15920	3398723..3398932	+	210	WP_003115568.1	cold-shock protein	-
JQM61_RS15925	3398993..3400867	-	1875	WP_003117607.1	MFS transporter	-
JQM61_RS15930	3401042..3403207	-	2166	WP_003091606.1	TonB-dependent receptor family protein	-
JQM61_RS15935	3403304..3404158	-	855	WP_012613852.1	AraC family transcriptional regulator	Antitoxin
JQM61_RS15940	3404225..3404812	-	588	WP_012613851.1	GNAT family N-acetyltransferase	Toxin
JQM61_RS15945	3405078..3408557	+	3480	WP_023115144.1	hybrid sensor histidine kinase/response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T190069 WP_012613851.1 NZ_CP069198:c3404812-3404225 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAVLEASFTG

Nucleotide

• Length: 588 bp

>T190069 NZ_CP091068:2016101-2016203 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31959.27 Da
• Isoelectric Point: 7.3276

>AT190069 WP_012613852.1 NZ_CP069198:c3404158-3403304 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLPVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT190069 NZ_CP091068:c2016210-2016065 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT