Detailed information of TA system TA190065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 2887285..2887850
• Replicon: chromosome
• Accession: NZ_CP069195
• Organism: Vibrio diabolicus strain SLV18

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: JOS67_RS13745
• Protein ID: WP_203346704.1
• Coordinates: 2887285..2887680 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: JOS67_RS13750
• Protein ID: WP_005397970.1
• Coordinates: 2887680..2887850 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JOS67_RS13735	2883079..2885169	-	2091	WP_203347274.1	YjbH domain-containing protein	-
JOS67_RS13740	2886104..2886522	-	419	Protein_2504	IS110 family transposase	-
JOS67_RS13745	2887285..2887680	-	396	WP_203346704.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JOS67_RS13750	2887680..2887850	-	171	WP_005397970.1	hypothetical protein	Antitoxin
JOS67_RS13755	2888566..2888727	-	162	WP_203346705.1	thrombospondin type 3 repeat-containing protein	-
JOS67_RS13760	2888749..2889180	-	432	WP_203346706.1	hypothetical protein	-
JOS67_RS13765	2889223..2890458	-	1236	WP_065275047.1	type II toxin-antitoxin system HipA family toxin	-
JOS67_RS13770	2890451..2890726	-	276	WP_065274105.1	helix-turn-helix domain-containing protein	-
JOS67_RS13775	2891289..2892635	-	1347	WP_203346707.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ugd	2859745..2908940	49195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14335.42 Da
• Isoelectric Point: 3.8306

>T190065 WP_203346704.1 NZ_CP069195:c2887680-2887285 [Vibrio diabolicus]
MDIICFPFERVIEINSFILATEPGMQGEVDIPKLQGALGRIDNAIAYDGLEDVFDIAAKYAASIAIAHALPDANKRTGLM
VALEYLSLNEFEIVIDNALLADAVRDLLLGVLTEADFADILYAQYTEGLER

Nucleotide

• Length: 396 bp

>T190065 NZ_CP091067:c6557534-6557352 [Pseudomonas sp. BC42]
ATGCGCAGTCGGGAGATGATCGGCAAGATTGAGGCGGATGGTTGGTACCTGATTGCCGTGAAGGGCAGTCACCATCAGTA
CAAGCATCCCTGGAAACCAGGGCGCGTCACTTTCAAGCATCCGGATGCGGAACTGCCCAGGGGCACGCTCAACAGCATCT
TGAAACAGGCGGGCTTGAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6314.48 Da
• Isoelectric Point: 11.4763

>AT190065 WP_005397970.1 NZ_CP069195:c2887850-2887680 [Vibrio diabolicus]
MSKDLSAYGVPQVKRPKVKATKQLDLSGMQGRQIVRSEAKLALRTHRKTFTKLADM

Nucleotide

• Length: 171 bp

>AT190065 NZ_CP091067:c6557322-6556921 [Pseudomonas sp. BC42]
ATGAAATTCCCGGTGGTTCTGCACAAGGATGCCGACTCTGAATACGGGGTGATCGTTCCGGATGTGCCGGGCTGTTACTC
CGCTGGTCACACGGTGTCCCTGGCGTTCGAGAACGTGAAGGAGGCGTTGTCCCTGCATTACGCAGGGCTGGTGGCCGATG
GCGAGCCTCTGCCGCAGGTGCACGAGATCGATGAGCACCTGGACAACCCCGACTACGCCGGGGGTGTATGGGGCGTGGTG
GAGTTCGACGTGACGCCGTATTTCGGCAAGTCGGTGCGCTTCAATGCCACCCTGCCGGAACACCTGCTGGAGCGCATCGA
TCAGACGGTCAAGCGTGACCAGCGCTACAACTCGCGTTCCGGGTTCCTGGCCGCCGCGGCGCTGCGCGAACTGTCGGCCT
GA