Detailed information of TA system TA190058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2719454..2720025
• Replicon: chromosome
• Accession: NZ_CP069185
• Organism: Enterococcus faecalis strain L11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JO410_RS13155
• Protein ID: WP_002354774.1
• Coordinates: 2719454..2719795 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: JO410_RS13160
• Protein ID: WP_002354773.1
• Coordinates: 2719795..2720025 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JO410_RS13150	2715469..2719083	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
JO410_RS13155	2719454..2719795	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JO410_RS13160	2719795..2720025	-	231	WP_002354773.1	hypothetical protein	Antitoxin
JO410_RS13165	2720199..2720414	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
JO410_RS13170	2720553..2721545	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
JO410_RS13175	2721712..2722350	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
JO410_RS13180	2723035..2724651	+	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T190058 WP_002354774.1 NZ_CP069185:c2719795-2719454 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T190058 NZ_CP091066:c2465914-2465648 [Staphylococcus aureus]
ATGAGCAATTATACGGTTAAGATTAAAAATTCGGCGAAATCAGATTTAAAGAAAATAACACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATGATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGGCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT190058 WP_002354773.1 NZ_CP069185:c2720025-2719795 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT190058 NZ_CP091066:c2466171-2465914 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAGCATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812