Detailed information of TA system TA190051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4526877..4527919
• Replicon: chromosome
• Accession: NZ_CP069177
• Organism: Pseudomonas aeruginosa strain Z154

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: IS492_RS21015
• Protein ID: WP_003153636.1
• Coordinates: 4526877..4527452 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: IS492_RS21020
• Protein ID: WP_003050245.1
• Coordinates: 4527449..4527919 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS492_RS20990 (IS492_20965)	4523315..4524040	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
IS492_RS20995 (IS492_20970)	4524079..4524981	-	903	WP_003090158.1	CBASS oligonucleotide cyclase	-
IS492_RS21000 (IS492_20975)	4524981..4525481	-	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
IS492_RS21005 (IS492_20980)	4525478..4525948	-	471	WP_003090160.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
IS492_RS21010 (IS492_20985)	4525945..4526859	-	915	WP_016852809.1	AAA family ATPase	-
IS492_RS21015 (IS492_20990)	4526877..4527452	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
IS492_RS21020 (IS492_20995)	4527449..4527919	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
IS492_RS21025 (IS492_21000)	4528123..4528506	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
IS492_RS21030 (IS492_21005)	4528503..4528736	+	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
IS492_RS21035 (IS492_21010)	4528753..4529112	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
IS492_RS21040 (IS492_21015)	4529124..4529522	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
IS492_RS21045 (IS492_21020)	4529519..4530211	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
IS492_RS21050 (IS492_21025)	4530208..4531119	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
IS492_RS21055 (IS492_21030)	4531109..4532527	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4480879..4527919	47040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T190051 WP_003153636.1 NZ_CP069177:c4527452-4526877 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T190051 NZ_CP091066:1815659-1815754 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT190051 WP_003050245.1 NZ_CP069177:c4527919-4527449 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT190051 NZ_CP091066:c1815839-1815782 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Structures

Antitoxin

• AlphaFold DB: I3TV68