Detailed information of TA system TA190049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 2593329..2594587
• Replicon: chromosome
• Accession: NZ_CP069177
• Organism: Pseudomonas aeruginosa strain Z154

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IS492_RS12260
• Protein ID: WP_003163133.1
• Coordinates: 2593832..2594587 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: IS492_RS12255
• Protein ID: WP_003086329.1
• Coordinates: 2593329..2593697 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS492_RS12235 (IS492_12230)	2589492..2589767	+	276	WP_010792073.1	zinc ribbon domain-containing protein	-
IS492_RS12240 (IS492_12235)	2589770..2590246	-	477	WP_031652752.1	hypothetical protein	-
IS492_RS12245 (IS492_12240)	2590421..2591914	+	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
IS492_RS12250 (IS492_12245)	2591970..2593256	+	1287	WP_003163134.1	FAD-binding oxidoreductase	-
IS492_RS12255 (IS492_12250)	2593329..2593697	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
IS492_RS12260 (IS492_12255)	2593832..2594587	+	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
IS492_RS12265 (IS492_12260)	2595356..2596717	+	1362	WP_078465093.1	DNA recombination protein RmuC	-
IS492_RS12270 (IS492_12265)	2596872..2599415	-	2544	WP_003140632.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T190049 WP_003163133.1 NZ_CP069177:2593832-2594587 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T190049 NZ_CP091066:c1488511-1488266 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT190049 WP_003086329.1 NZ_CP069177:2593329-2593697 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT190049 NZ_CP091066:1487932-1488297 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Antitoxin

• AlphaFold DB: V6AJU7