Detailed information of TA system TA190047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1742767..1743353
• Replicon: chromosome
• Accession: NZ_CP069177
• Organism: Pseudomonas aeruginosa strain Z154

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: IS492_RS08135
• Protein ID: WP_003120987.1
• Coordinates: 1742767..1743066 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IS492_RS08140
• Protein ID: WP_003448662.1
• Coordinates: 1743063..1743353 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS492_RS08115 (IS492_08105)	1737880..1738413	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
IS492_RS08120 (IS492_08110)	1738403..1739728	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
IS492_RS08125 (IS492_08115)	1739732..1741441	-	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
IS492_RS08130 (IS492_08120)	1741441..1742565	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
IS492_RS08135 (IS492_08125)	1742767..1743066	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IS492_RS08140 (IS492_08130)	1743063..1743353	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
IS492_RS08145 (IS492_08135)	1743424..1743768	-	345	WP_016851612.1	hypothetical protein	-
IS492_RS08150 (IS492_08140)	1743909..1745885	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
IS492_RS08155 (IS492_08145)	1745882..1747771	-	1890	WP_016851610.1	hypothetical protein	-
IS492_RS08160 (IS492_08150)	1747993..1748202	-	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1685662..1769876	84214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T190047 WP_003120987.1 NZ_CP069177:1742767-1743066 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T190047 NZ_CP091065:c4568827-4568486 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT190047 WP_003448662.1 NZ_CP069177:1743063-1743353 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT190047 NZ_CP091065:4568822-4568898 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: G8CP73