Detailed information of TA system TA190044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1137..1611
• Replicon: plasmid pF
• Accession: NZ_CP069175
• Organism: Klebsiella pneumoniae strain KPWX136

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: JLH18_RS29685
• Protein ID: WP_004197762.1
• Coordinates: 1137..1403 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: JLH18_RS29690
• Protein ID: WP_000879771.1
• Coordinates: 1387..1611 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH18_RS29675	106..651	+	546	WP_023317533.1	hypothetical protein	-
JLH18_RS29680	717..1067	+	351	WP_004197756.1	hypothetical protein	-
JLH18_RS29685	1137..1403	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLH18_RS29690	1387..1611	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
JLH18_RS29695	2278..2490	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
JLH18_RS29700	2497..3006	-	510	WP_032433972.1	MbeB family mobilization protein	-
JLH18_RS29705	2981..3745	-	765	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
JLH18_RS29710	3684..4028	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
JLH18_RS29715	4018..4194	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T190044 WP_004197762.1 NZ_CP069175:c1403-1137 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T190044 NZ_CP091065:4341895-4342068 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT190044 WP_000879771.1 NZ_CP069175:c1611-1387 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT190044 NZ_CP091065:4341571-4341867 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: S1EJF5