Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 180056..180966 Replicon plasmid pA
Accession NZ_CP069171
Organism Klebsiella pneumoniae strain KPWX136

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag JLH18_RS27990 Protein ID WP_004026354.1
Coordinates 180496..180966 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag JLH18_RS27985 Protein ID WP_004026357.1
Coordinates 180056..180499 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLH18_RS27960 175304..176385 + 1082 Protein_190 DEAD/DEAH box helicase -
JLH18_RS27965 176610..176831 - 222 WP_004213615.1 hypothetical protein -
JLH18_RS27970 176893..178200 - 1308 Protein_192 FepA family TonB-dependent siderophore receptor -
JLH18_RS27975 178266..179246 + 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
JLH18_RS27980 179452..179955 + 504 Protein_194 DUF4113 domain-containing protein -
JLH18_RS27985 180056..180499 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
JLH18_RS27990 180496..180966 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
JLH18_RS27995 181115..181812 + 698 Protein_197 IS1 family transposase -
JLH18_RS28000 181829..182263 + 435 Protein_198 thioredoxin fold domain-containing protein -
JLH18_RS28005 182280..182591 + 312 WP_011251282.1 hypothetical protein -
JLH18_RS28010 182605..182946 + 342 WP_011251281.1 hypothetical protein -
JLH18_RS28015 183006..183962 + 957 WP_011251280.1 DsbA family protein -
JLH18_RS28020 184387..184827 - 441 WP_011251275.1 hypothetical protein -
JLH18_RS28025 184817..185327 - 511 Protein_203 hypothetical protein -
JLH18_RS28030 185336..185650 - 315 WP_011251273.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / iroN 1..191359 191359
- inside IScluster/Tn - iroN 173655..187247 13592


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T190033 WP_004026354.1 NZ_CP069171:180496-180966 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T190033 NZ_CP091065:1777648-1777751 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT190033 WP_004026357.1 NZ_CP069171:180056-180499 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT190033 NZ_CP091065:c1777789-1777518 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References