Detailed information of TA system TA190032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 146601..147271
• Replicon: plasmid pA
• Accession: NZ_CP069171
• Organism: Klebsiella pneumoniae strain KPWX136

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: JLH18_RS27790
• Protein ID: WP_004213072.1
• Coordinates: 146828..147271 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: JLH18_RS27785
• Protein ID: WP_004213073.1
• Coordinates: 146601..146831 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH18_RS27760	142824..143723	-	900	WP_004225022.1	nucleotide-binding protein	-
JLH18_RS27765	143713..144003	-	291	WP_004213078.1	hypothetical protein	-
JLH18_RS27770	144355..144561	+	207	WP_004213077.1	hypothetical protein	-
JLH18_RS27775	144551..144844	-	294	WP_004213076.1	hypothetical protein	-
JLH18_RS27780	144860..145993	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
JLH18_RS27785	146601..146831	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JLH18_RS27790	146828..147271	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLH18_RS27795	147285..147701	-	417	Protein_157	restriction endonuclease	-
JLH18_RS27800	147705..147998	-	294	Protein_158	transposase	-
JLH18_RS27805	148415..149050	+	636	Protein_159	response regulator	-
JLH18_RS27810	149565..149971	-	407	Protein_160	GAF domain-containing protein	-
JLH18_RS27815	150062..150982	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
JLH18_RS27820	151031..151522	-	492	WP_011251327.1	DM13 domain-containing protein	-
JLH18_RS27825	151585..151860	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroN	1..191359	191359

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T190032 WP_004213072.1 NZ_CP069171:146828-147271 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T190032 NZ_CP091065:c1624062-1623688 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT190032 WP_004213073.1 NZ_CP069171:146601-146831 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT190032 NZ_CP091065:c1624519-1624151 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620