Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4478282..4478898 Replicon chromosome
Accession NZ_CP069159
Organism Citrobacter sp. R56

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag JM656_RS21025 Protein ID WP_192613908.1
Coordinates 4478282..4478656 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag JM656_RS21030 Protein ID WP_203359404.1
Coordinates 4478656..4478898 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JM656_RS21010 4475784..4476686 + 903 WP_192613905.1 formate dehydrogenase subunit beta -
JM656_RS21015 4476683..4477318 + 636 WP_192613906.1 formate dehydrogenase cytochrome b556 subunit -
JM656_RS21020 4477315..4478244 + 930 WP_192613907.1 formate dehydrogenase accessory protein FdhE -
JM656_RS21025 4478282..4478656 - 375 WP_192613908.1 type II toxin-antitoxin system VapC family toxin Toxin
JM656_RS21030 4478656..4478898 - 243 WP_203359404.1 ribbon-helix-helix domain-containing protein Antitoxin
JM656_RS21035 4479098..4480006 + 909 WP_203359405.1 alpha/beta hydrolase -
JM656_RS21040 4480067..4480180 + 114 WP_203361347.1 type II toxin-antitoxin system ParD family antitoxin -
JM656_RS21045 4480189..4481130 - 942 WP_203359406.1 bifunctional GNAT family N-acetyltransferase/hotdog fold thioesterase -
JM656_RS21050 4481175..4481612 - 438 WP_008501836.1 D-tyrosyl-tRNA(Tyr) deacylase -
JM656_RS21055 4481609..4482481 - 873 WP_203359407.1 virulence factor BrkB family protein -
JM656_RS21060 4482475..4483074 - 600 WP_203359408.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-49)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13753.91 Da        Isoelectric Point: 7.2024

>T190007 WP_192613908.1 NZ_CP069159:c4478656-4478282 [Citrobacter sp. R56]
MAKGSALFDTNILIDLFSGRGEAKQALDAWPPQNAISLITWMEVLVGARKYNQEHRTRVAMSAFNIIGVSQEIAERSVAL
RQEYAMKLPDAIILATAQIHRLELVTRNTKDFAGIPGVVTPYEL

Download         Length: 375 bp

>T190007 NZ_CP091059:c157780-157337 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9349.64 Da        Isoelectric Point: 4.3894

>AT190007 WP_203359404.1 NZ_CP069159:c4478898-4478656 [Citrobacter sp. R56]
MSIMAGLDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQSNTTISNALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT190007 NZ_CP091059:c158007-157777 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References