Detailed information of TA system TA190005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3957591..3958167
• Replicon: chromosome
• Accession: NZ_CP069159
• Organism: Citrobacter sp. R56

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: JM656_RS18675
• Protein ID: WP_203359047.1
• Coordinates: 3957880..3958167 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: JM656_RS18670
• Protein ID: WP_203359046.1
• Coordinates: 3957591..3957893 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JM656_RS18650	3953292..3953804	+	513	WP_203359044.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
JM656_RS18655	3954076..3956226	+	2151	WP_203359045.1	pyruvate/proton symporter BtsT	-
JM656_RS18660	3956323..3956517	+	195	WP_192610940.1	YbdD/YjiX family protein	-
JM656_RS18665	3956530..3957486	+	957	WP_192610939.1	GTPase	-
JM656_RS18670	3957591..3957893	-	303	WP_203359046.1	BrnA antitoxin family protein	Antitoxin
JM656_RS18675	3957880..3958167	-	288	WP_203359047.1	BrnT family toxin	Toxin
JM656_RS18680	3958547..3959131	-	585	WP_203359048.1	OmpA family protein	-
JM656_RS18685	3959135..3959611	-	477	WP_203359049.1	hypothetical protein	-
JM656_RS18690	3960031..3960864	+	834	WP_203359050.1	xanthosine phosphorylase	-
JM656_RS18695	3960910..3962109	+	1200	WP_203359051.1	MFS transporter TsgA	-
JM656_RS18700	3962166..3963083	+	918	WP_203361327.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11149.61 Da
• Isoelectric Point: 7.4758

>T190005 WP_203359047.1 NZ_CP069159:c3958167-3957880 [Citrobacter sp. R56]
MPMEYEWDANKARSNQIKHGIRFEDAVLVFDDPRHLSRQDRIENGEHRWQTLGLVHGIIVILVAHTVRFESGCEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T190005 NZ_CP091059:c58146-57835 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11410.00 Da
• Isoelectric Point: 9.9265

>AT190005 WP_203359046.1 NZ_CP069159:c3957893-3957591 [Citrobacter sp. R56]
MSMVKHKRGSVSALSAQHEAELKALAMKSDTDIDYSDIPSSEDEQWSEAARGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT190005 NZ_CP091059:c57838-57419 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA